Retention of eyeblink conditioning in periweanling and adult rats

Eyeblink conditioning is a well-established model for studying the developmental neurobiology of associative learning and memory, though nothing is known regarding developmental differences in retention between periweanling and adult rats. The present study examined retention of eyeblink conditioning in periweanling (postnatal day 24 at the start of training) and adult rats 1, 7, or 28 days after acquisition. Retention was assessed by (1) a conditional stimulus (CS)-alone test session followed by (2) CS-unconditional stimulus (US) reacquisition tests. Conditional response (CR) levels at acquisition were comparable in most respects between ages, and robust CR levels were present at the start of retention tests for both ages in the 1 day group, with CR percentages at block 1 of reacquisition higher in periweanlings relative to adults. At the 7 day retention test there was a trend toward significance for higher CR percentages at the CS-alone test in adults relative to periweanlings, though there were no age differences at reacquisition testing. When testing occurred 28 days after acquisition, however, periweanlings showed fewer CRs relative to adults during reacquisition despite low CR levels in both ages throughout the CS-alone test. Furthermore, periweanlings in the 28 day group required more trials at reacquisition than all other groups to exceed CR levels from their first acquisition session. These findings are consistent with rapid forgetting in the young commonly referred to as "infantile amnesia." The well-characterized eyeblink preparation may be useful for future studies investigating neural mechanisms responsible for rapid forgetting in developing animals.