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Transcriptome analysis of aging mouse meibomian glands.

Geraint J Parfitt1, Donald J Brown1, James V Jester1

  • 1The Gavin Herbert Eye Institute, School of Medicine, University of California, Irvine, CA.

Molecular Vision
|June 10, 2016
PubMed
Summary

Aging meibomian glands show altered Wnt and fibroblast growth factor (FGF) signaling pathways. This study reveals key molecular changes in age-related meibomian gland dysfunction (ARMGD) in mice.

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Area of Science:

  • Ophthalmology
  • Genomics
  • Aging Research

Background:

  • Dry eye disease is linked to age-related meibomian gland dysfunction (ARMGD).
  • ARMGD in old mice mirrors human meibomian gland dysfunction (MGD).

Purpose of the Study:

  • To investigate the molecular mechanisms underlying ARMGD.
  • To generate transcriptome profiles of eyelids from young and old mice.

Main Methods:

  • RNA sequencing of eyelid tissues from male and female C57BL/6 mice (3 months vs. 2 years old).
  • Differential gene expression analysis using CyberT.
  • Mapping reads to the mm10 reference genome.

Main Results:

  • Approximately 55 million reads per library, with 15,000 genes expressed.
  • 698 differentially expressed genes identified between young and old mice.
  • Gene Ontology analysis highlighted altered cellular, developmental, and metabolic processes, notably Wnt signaling.

Conclusions:

  • RNA sequencing identified altered fibroblast growth factor (FGF) and Wnt signaling pathways in aging mouse meibomian glands.
  • These findings provide insights into the molecular basis of ARMGD.