Related Experiment Video
Updated: Mar 19, 2026
08:41
Surgical Procedures and Methodology for a Preclinical Murine Model of De Novo Mammary Cancer Metastasis
Published on: July 29, 2017
14.7K
Feline Mammary Cancer.
B B Hassan1,2, S M Elshafae1,3, W Supsavhad1
11 Department of Veterinary Biosciences, College of Veterinary Medicine, The Ohio State University, Columbus, OH, USA.
Veterinary Pathology
|June 10, 2016
Summary
Feline mammary carcinoma (FMC) models in nude mice mimic human breast cancer progression. This study identified key genes involved in FMC metastasis, offering potential therapeutic targets for aggressive breast cancers.
Area of Science:
- Comparative oncology
- Molecular biology
- Cancer research
Background:
- Feline mammary carcinoma (FMC) shares similarities with human breast cancer, including late onset and metastatic behavior.
- FMC is a valuable model for studying aggressive human breast cancer due to these parallels.
Purpose of the Study:
- To establish a nude mouse model for FMC tumor growth and metastasis.
- To investigate gene expression related to lymphangiogenesis, angiogenesis, tumor progression, and metastasis in FMC.
Main Methods:
- Subcutaneous and direct injection (intratibial, intracardiac) of FMC tissues and cell lines into nude mice.
- Monitoring tumor growth and metastasis using bioluminescent imaging, necropsy, radiology, and histopathology.
- Quantitative real-time reverse transcription polymerase chain reaction (qRT-PCR) for gene expression analysis in FMC tissues, xenografts, and cell lines.
Main Results:
- Intratibial and intracardiac injections successfully induced metastasis to lung, brain, liver, kidney, eye, and bone.
- Histopathology of subcutaneous xenografts confirmed resemblance to primary FMC tumors.
- Fifteen genes, including PDGFA, PDGFB, PDGFC, FGF2, EGFR, ERBB2, ERBB3, VEGFD, VEGFR3, and MYOF, showed differential expression in FMC samples.
Conclusions:
- Nude mouse models are effective for studying FMC progression and metastasis.
- Identified differentially expressed genes provide potential molecular targets for FMC treatment and understanding aggressive breast cancer biology.
