Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Inhibitory role of G<sub>i</sub>-coupled receptors on cAMP-driven cancers with focus on opioid receptors in lung adenocarcinoma and its stem cells.

Vitamins and hormones·2019
Same author

The impact of smoking and the influence of other factors on lung cancer.

Expert review of respiratory medicine·2019
Same author

Biphasic Effect of Sildenafil on Energy Sensing is Mediated by Phosphodiesterases 2 and 3 in Adipocytes and Hepatocytes.

International journal of molecular sciences·2019
Same author

The Neuro-Psychological Axis of Smoking-Associated Cancer.

Journal of immunological sciences·2019
Same author

Repurposing established cyclic adenosine monophosphate reducing agents for the prevention and therapy of epidermal growth factor receptor inhibitor resistance in non-small cell lung cancer.

Translational lung cancer research·2018
Same author

Erratum: Leucine-nicotinic acid synergy stimulates AMPK/Sirt1 signaling and regulates lipid metabolism and lifespan in Caenorhabditis elegans, and hyperlipidemia and atherosclerosis in mice.

American journal of cardiovascular disease·2017

Related Experiment Video

Updated: Mar 19, 2026

Studying Pancreatic Cancer Stem Cell Characteristics for Developing New Treatment Strategies
07:29

Studying Pancreatic Cancer Stem Cell Characteristics for Developing New Treatment Strategies

Published on: June 20, 2015

20.3K

Prevention of pancreatic cancer in a hamster model by cAMP decrease.

Jheelam Banerjee1, Arokya M S Papu John1, Mohammed H Al-Wadei1

  • 1Experimental Oncology Laboratory, Department of Biomedical & Diagnostic Sciences, College of Veterinary Medicine, University of Tennessee, Knoxville, TN, USA.

Oncotarget
|June 10, 2016
PubMed
Summary

Gamma-aminobutyric acid (GABA) supplementation may prevent pancreatic cancer. This study found GABA reduced cyclic adenosine monophosphate (cAMP) and key cancer markers in a hamster model, offering a potential prevention strategy for high-risk individuals.

Keywords:
cancer stem cell signalingcyclic adenosine monophosphatepancreatic cancerpreventionγ-aminobutyric acid

More Related Videos

An Orthotopic Resectional Mouse Model of Pancreatic Cancer
07:17

An Orthotopic Resectional Mouse Model of Pancreatic Cancer

Published on: September 24, 2020

12.9K
An Obstructive Chronic Pancreatitis Model Established Through Electrocoagulation
06:28

An Obstructive Chronic Pancreatitis Model Established Through Electrocoagulation

Published on: October 31, 2025

541

Related Experiment Videos

Last Updated: Mar 19, 2026

Studying Pancreatic Cancer Stem Cell Characteristics for Developing New Treatment Strategies
07:29

Studying Pancreatic Cancer Stem Cell Characteristics for Developing New Treatment Strategies

Published on: June 20, 2015

20.3K
An Orthotopic Resectional Mouse Model of Pancreatic Cancer
07:17

An Orthotopic Resectional Mouse Model of Pancreatic Cancer

Published on: September 24, 2020

12.9K
An Obstructive Chronic Pancreatitis Model Established Through Electrocoagulation
06:28

An Obstructive Chronic Pancreatitis Model Established Through Electrocoagulation

Published on: October 31, 2025

541

Area of Science:

  • Oncology
  • Gastroenterology
  • Neuroscience

Background:

  • Smoking and alcoholism are established risk factors for pancreatic ductal adenocarcinoma (PDAC).
  • PDAC is associated with overexpression of stress neurotransmitters and cyclic adenosine monophosphate (cAMP), and suppressed gamma-aminobutyric acid (GABA).
  • Previous research indicated a link between neurotransmitter imbalance and PDAC development.

Purpose of the Study:

  • To test if decreasing cAMP levels through GABA supplementation can prevent pancreatitis-associated PDAC.
  • To evaluate the efficacy of GABA as a preventive agent against PDAC and pancreatic intraductal neoplasia (PanIN).

Main Methods:

  • A hamster model was utilized to study the effects of GABA supplementation in drinking water.
  • Enzyme-linked immunosorbent assay (ELISA) and immunohistochemistry were employed to measure molecular changes.
  • Analysis focused on cAMP levels, interleukin-6, cancer stem cell markers, and phosphorylated signaling proteins.

Main Results:

  • GABA supplementation demonstrated significant preventive effects against PDAC and PanIN development.
  • Treated animals showed reduced levels of cAMP and interleukin-6.
  • Expression of cancer stem cell markers and proteins promoting proliferation and migration were significantly decreased.

Conclusions:

  • Decreasing cAMP levels via GABA supplementation effectively inhibits multiple cancer-promoting pathways.
  • GABA acts on cancer stem cells, differentiated cancer cells, and the immune system to inhibit PDAC.
  • GABA supplementation represents a promising novel strategy for PDAC prevention in high-risk populations, including smokers and alcohol consumers.