Related Experiment Video

Updated: Mar 19, 2026

Using Microtiter Dish Radiolabeling for Multiple In Vivo Measurements Of Escherichia coli pppGpp Followed by Thin Layer Chromatography
06:30

Using Microtiter Dish Radiolabeling for Multiple In Vivo Measurements Of Escherichia coli pppGpp Followed by Thin Layer Chromatography

Published on: June 4, 2019

6.5K

[THE ROLE OF (p)ppGpp MOLECULES IN FORMATION OF "STRICT RESPONSE" IN BACTERIA AND BIOSYNTHESIS OF ANTIBIOTICS AND

D Klymyshin, O Stephanyshyn, V Fedorenko

    Tsitologiia I Genetika
    |June 11, 2016
    PubMed

    More Related Videos

    A Purification and In Vitro Activity Assay for a pppGpp Synthetase from Clostridium difficile09:53

    A Purification and In Vitro Activity Assay for a pppGpp Synthetase from Clostridium difficile

    Published on: November 3, 2018

    9.0K
    Functional Complementation Analysis FCA: A Laboratory Exercise Designed and Implemented to Supplement the Teaching of Biochemical Pathways09:27

    Functional Complementation Analysis FCA: A Laboratory Exercise Designed and Implemented to Supplement the Teaching of Biochemical Pathways

    Published on: June 24, 2016

    18.3K

    Related Concept Videos

    Stringent Response in E. coli01:23

    Stringent Response in E. coli

    Bacterial growth is closely tied to nutrient availability, with cells proliferating exponentially under favorable conditions and entering a stationary phase when resources become scarce. This transition is mediated by a regulatory mechanism known as the stringent response, which allows bacteria to adapt to nutrient deprivation by modulating gene expression and metabolic activity.During nutrient scarcity, intracellular amino acid levels decline. It results in the accumulation of uncharged tRNAs...
    457
    Global Regulatory Systems01:28

    Global Regulatory Systems

    Global regulatory systems in bacteria enable rapid and coordinated responses to environmental changes by integrating sensory inputs with gene expression, ensuring efficient adaptation to fluctuating conditions. Key global regulatory mechanisms include regulons, two-component systems, sigma factors, and secondary messengers.Regulons and Global RegulatorsA regulon is a collection of genes and operons controlled by a common global regulator. These regulators enable bacteria to prioritize resource...
    845
    Prokaryotic Transcriptional Activators and Repressors01:58

    Prokaryotic Transcriptional Activators and Repressors

    The organization of prokaryotic genes in their genome is notably different from that of eukaryotes. Prokaryotic genes are organized, such that the genes for proteins involved in the same biochemical process or function are located together in groups. This group of genes, along with their regulatory elements, are collectively known as an operon. The functional genes in an operon are transcribed together to give a single strand of mRNA known as polycistronic mRNA.
    Transcription of prokaryotic...
    26.2K
    Prokaryotic Transcriptional Activators and Repressors01:58

    Prokaryotic Transcriptional Activators and Repressors

    11.3K
    Gene Regulation in Microbial Communities: Quorum Sensing01:28

    Gene Regulation in Microbial Communities: Quorum Sensing

    Quorum sensing is a mechanism of bacterial communication that enables coordinated gene expression in response to changes in population density. This facilitates collective behaviors that enhance survival, resource acquisition, and ecological adaptation. This process relies on small signaling molecules called autoinducers that accumulate as bacterial populations grow. When a critical threshold concentration of autoinducers is reached, bacterial cells collectively modify gene expression,...
    849
    Regulation of Bacterial Virulence01:28

    Regulation of Bacterial Virulence

    Pathogenic bacteria employ a range of regulatory mechanisms to modulate the expression of virulence genes in response to environmental and host-derived signals. These mechanisms ensure that virulence factors are expressed only under favorable conditions, thereby optimizing infection and survival strategies.Mechanisms of Virulence RegulationKey regulatory strategies include:Two-Component Systems: These consist of a membrane-bound sensor kinase and a cytoplasmic response regulator. Environmental...
    3