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Characterization of GPR101 transcript structure and expression patterns.

Giampaolo Trivellin1, Ivana Bjelobaba2, Adrian F Daly3

  • 1Section on Endocrinology and GeneticsEunice Kennedy Shriver National Institute of Child Health and Human Development (NICHD), National Institutes of Health (NIH), Bethesda, Maryland, USA.

Journal of Molecular Endocrinology
|June 11, 2016
PubMed
Summary
This summary is machine-generated.

GPR101 gene expression varies significantly across human development and species. Primarily found in the brain, its pituitary expression is crucial during fetal and adolescent stages, suggesting a role in development.

Keywords:
GPR101expression analysishypothalamuspituitarytranscripts structure

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Area of Science:

  • Genetics
  • Endocrinology
  • Developmental Biology

Background:

  • X-linked acrogigantism (X-LAG) is caused by Xq26.3 microduplications, often involving pituitary adenomas.
  • GPR101 is highly expressed in X-LAG pituitary lesions, but its expression patterns are poorly understood.

Purpose of the Study:

  • To characterize GPR101 transcripts and expression patterns across human tissues and species.
  • To investigate the role of GPR101 in brain and pituitary development.

Main Methods:

  • 5'-Rapid Amplification of cDNA Ends (RACE) and RNA sequencing for transcript characterization.
  • RT-quantitative PCR (qPCR), whole-mount in situ hybridization, and immunostaining for expression analysis.
  • Bioinformatic prediction of the GPR101 promoter region.

Main Results:

  • Identified four GPR101 isoforms with distinct 5'-untranslated regions (UTRs) and a common 3'UTR.
  • GPR101 expression is low in most adult human tissues but present in specific brain regions.
  • Human pituitary GPR101 expression is high in fetuses and adolescents, decreasing in adults; expression differs in adult monkey and rat pituitaries.
  • Gpr101 shows developmental and sexually dimorphic expression in rat pituitary and is present in developing rat and zebrafish brain and pituitary.

Conclusions:

  • GPR101 exhibits diverse transcript isoforms and is predominantly expressed in the brain across species.
  • Species- and temporal-specific expression patterns of GPR101 suggest a significant role in brain and pituitary development.
  • Expression differences may reflect varying growth and maturation patterns among species.