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Super-resolution Fluorescence Microscopy

Super-resolution Fluorescence Microscopy

Super-resolution fluorescence microscopy (SRFM) provides a better resolution than conventional fluorescence microscopy by reducing the point spread function (PSF). PSF is the light intensity distribution from a point that causes it to appear blurred. Due to PSF, each fluorescing point appears bigger than its actual size, and it is the PSF interference of nearby fluorophores that causes the blurred image. Various approaches to achieving higher resolution through SRFM have recently been...

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Related Experiment Video

Updated: Mar 19, 2026

Multicolor Fluorescence Detection for Droplet Microfluidics Using Optical Fibers

Multicolor Fluorescence Detection for Droplet Microfluidics Using Optical Fibers

Published on: May 5, 2016

Droplet Enhanced Fluorescence for Ultrasensitive Detection Using Inkjet.

Hulie Zeng¹, Daisuke Katagiri¹, Taisuke Ogino¹

¹Department of Applied Chemistry, Graduate School of Urban Environmental Sciences, Tokyo Metropolitan University , 1-1 Minamiohsawa, Hachioji, Tokyo 192-0397, Japan.

Analytical Chemistry

|June 11, 2016

Summary

Researchers developed droplet enhanced fluorescence (DEF) for ultrasensitive detection in microliter liquid samples. This method achieved low detection limits for fluorescein sodium and single-stranded DNA, offering a new technique for sensitive sample analysis.

Area of Science:

Analytical Chemistry
Optics and Photonics
Biotechnology

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Last Updated: Mar 19, 2026

Multicolor Fluorescence Detection for Droplet Microfluidics Using Optical Fibers

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Fluorescence detection methods for microfluidic droplet platforms

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Published on: July 10, 2018