Related Experiment Video
Updated: Mar 19, 2026

Single-Molecule Imaging of Nuclear Transport
Published on: June 9, 2010
Single-Molecule Imaging to Characterize the Transport Mechanism of the Nuclear Pore Complex
Grace Jeremy1, James Stevens2, Alan R Lowe3,4,5
1Structural & Molecular Biology, University College London, Gower Street, London, WC1E 6BT, UK.
Abstract:
In the eukaryotic cell, a large macromolecular channel, known as the Nuclear Pore Complex (NPC), mediates all molecular transport between the nucleus and cytoplasm. In recent years, single-molecule fluorescence (SMF) imaging has emerged as a powerful tool to study the molecular mechanism of transport through the NPC. More recently, techniques such as single-molecule localization microscopy (SMLM) have enabled the spatial and temporal distribution of cargos, transport receptors and even structural components of the NPC to be determined with nanometre accuracy. In this protocol, we describe a method to study the position and/or motion of individual molecules transiting through the NPC with high spatial and temporal precision.
More Related Videos
11:55Membrane Transport Processes Analyzed by a Highly Parallel Nanopore Chip System at Single Protein Resolution
Published on: August 16, 2016
08:55Single-Molecule Imaging of Lateral Mobility and Ion Channel Activity in Lipid Bilayers using Total Internal Reflection Fluorescence TIRF Microscopy
Published on: February 17, 2023
Related Concept Videos
Nuclear Protein Sorting
Proteins targeted to the nucleus carry nuclear localization signals or NLS recognized by import receptors in the cytosol. Similarly, proteins with nuclear export signals are recognized by export receptors. Import and export receptors are...
Directionality of Nuclear Transport
Regulation of Nuclear Protein Sorting
Nuclear Localization Signals and Import
Nuclear Export
NES are of three types- the canonical 10-residue long leucine-rich signal and other...