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Noninvasive Imaging of Fluorescent Reporters in Small Rodent Models Using Fluorescence Molecular Tomography.

Weizhou Hou1, Steve H Thorne2

  • 1Department of Radiology, Hillman Cancer Center, University of Pittsburgh, Suite 1.46e, 5117 Centre Avenue, Pittsburgh, PA, 15213, USA.

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|June 11, 2016
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Summary

Noninvasive fluorescence imaging allows researchers to study molecular events in live animals during preclinical research. Careful consideration of reporters, models, and systems is crucial for successful small animal imaging studies.

Keywords:
FluorescenceMolecular imagingNoninvasiveOpticalReportersWhole animal

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Area of Science:

  • Biomedical imaging
  • Preclinical research
  • Molecular biology

Background:

  • Fluorescence imaging is a powerful tool for studying molecular events in live animals.
  • Noninvasive whole animal imaging combines genetic reporters and probes.
  • Light properties in tissues necessitate careful experimental design.

Purpose of the Study:

  • To describe approaches for designing and conducting experiments using noninvasive whole animal fluorescence imaging.
  • To guide researchers in incorporating fluorescence imaging into small animal studies.
  • To address challenges related to light adsorption and diffraction in tissue imaging.

Main Methods:

  • Utilizing genetic reporters for endogenous molecular events.
  • Employing exogenously added probes for targeted molecular detection.
  • Implementing noninvasive whole animal fluorescence imaging techniques.
  • Careful selection of imaging systems and small animal models.

Main Results:

  • Demonstrated feasibility of noninvasive whole animal fluorescence imaging in small animals.
  • Provided a framework for experimental design considering optical properties of tissues.
  • Highlighted the importance of choosing appropriate reporters and probes.

Conclusions:

  • Noninvasive fluorescence imaging is a valuable technique for preclinical research.
  • Successful implementation requires careful consideration of experimental parameters.
  • This approach facilitates the investigation of molecular processes in live subjects.