Ex Vivo Imaging, Biodistribution, and Histological Study in Addition to In Vivo Imaging

In addition to in vivo fluorescence imaging, ex vivo evaluations including ex vivo imaging, biodistribution, and histological study are often conducted to further investigate the biological behavior of fluorescent probes. These studies can further confirm the localization of fluorescent probes at the target sites and demonstrate the probe distribution in various organs and tissues. Such studies can also be extended to cellular level for biochemical analysis. Therefore, ex vivo evaluations are valuable to fully characterizing fluorescent probes in a living system. This chapter provides an overview of techniques for evaluating pharmacological profiles of fluorescent probes ex vivo.