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Moxifloxacin: Clinically compatible contrast agent for multiphoton imaging.

Taejun Wang1, Won Hyuk Jang1, Seunghun Lee2

  • 1Division of Integrative Biosciences and Biotechnology, Pohang University of Science and Technology, 77 Cheongam-ro, Nam-gu, Pohang, Gyeongbuk 37673, Rep. of Korea.

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Moxifloxacin, an antibiotic, offers bright intrinsic fluorescence for multiphoton microscopy (MPM) cell labeling. This technique enhances imaging speed and applicability in biological and clinical studies of human tissues.

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Area of Science:

  • Biomedical imaging
  • Cellular biology
  • Microscopy

Background:

  • Multiphoton microscopy (MPM) is crucial for deep tissue and live animal imaging.
  • MPM in human tissues is hindered by weak autofluorescence and probe cytotoxicity.
  • Novel cell-labeling agents are needed to overcome these limitations.

Purpose of the Study:

  • To evaluate moxifloxacin as a novel cell-labeling agent for MPM.
  • To assess moxifloxacin's suitability for imaging various cell lines and animal tissues.
  • To explore the potential of moxifloxacin-based MPM for clinical applications.

Main Methods:

  • Utilized moxifloxacin, an FDA-approved antibiotic, as an intrinsic fluorescent probe.
  • Applied MPM to label and image various cell lines (e.g., HeLa, fibroblasts).
  • Demonstrated MPM imaging in ex vivo animal tissues: cornea, skin, small intestine, and bladder.

Main Results:

  • Moxifloxacin exhibits bright intrinsic multiphoton fluorescence.
  • The agent shows good tissue penetration and high intracellular concentration.
  • MPM imaging with moxifloxacin achieved significantly faster acquisition times (10x) compared to endogenous fluorescence.

Conclusions:

  • Moxifloxacin is a promising cell-labeling agent for MPM.
  • Its intrinsic fluorescence and tissue penetration facilitate efficient cellular and tissue imaging.
  • Moxifloxacin-based MPM holds potential for accelerated clinical diagnostics and research.