Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Protein Dynamics in Living Cells

Protein Dynamics in Living Cells

Different fluorescence-based techniques are used to study the protein dynamics in living cells. These techniques include FRAP, FRET, and PET.
Fluorescent recovery after photobleaching (FRAP) is a fluorescent-protein-based detection technique used to quantify protein movement rates within the cell. This method exposes a small portion of the cell to an intense laser beam. The laser beam causes permanent photobleaching of the fluorophore-tagged proteins in the exposed region. As the bleached...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Distinct DNA repair mechanisms prevent formaldehyde toxicity during development, reproduction and aging.

Nucleic acids research·2024

Same author

Endogenous formaldehyde scavenges cellular glutathione resulting in redox disruption and cytotoxicity.

Nature communications·2022

Same author

Molecular pathology of rare progeroid diseases.

Trends in molecular medicine·2021

Same author

A C. elegans model for neurodegeneration in Cockayne syndrome.

Nucleic acids research·2020

Same author

Improved Biocompatibility of Amino-Functionalized Graphene Oxide in Caenorhabditis elegans.

Small (Weinheim an der Bergstrasse, Germany)·2019

Same author

Restoration of Proteostasis in the Endoplasmic Reticulum Reverses an Inflammation-Like Response to Cytoplasmic DNA in <i>Caenorhabditis elegans</i>.

Genetics·2019

Search research articles

Related Experiment Video

Updated: Mar 19, 2026

Author Spotlight: Photo Switchable Protein Recruitment for Reversible Patterning in Artificial Cellular Systems

Author Spotlight: Photo Switchable Protein Recruitment for Reversible Patterning in Artificial Cellular Systems

Published on: February 23, 2024

Light Sheet Microscopy to Measure Protein Dynamics.

Matthias Rieckher¹

¹Institute for Genome Stability in Ageing and Disease, Cologne Cluster of Excellence in Cellular Stress Responses in Aging-Associated Diseases (CECAD), University of Cologne, Cologne, Germany. m_rieckher@gmx.com, mrieckh1@uni-koeln.de.

Journal of Cellular Physiology

|June 11, 2016

Summary

This summary is machine-generated.

Visualizing protein dynamics requires advanced microscopy. Light sheet microscopy enables high-resolution, in vivo imaging of molecular mechanisms in living cells and whole organisms.

More Related Videos

Tracking Single Proteins in Lipid Bilayers Using Fluorescence Microscopy

Tracking Single Proteins in Lipid Bilayers Using Fluorescence Microscopy

Published on: December 12, 2025

Study of Protein Dynamics via Neutron Spin Echo Spectroscopy

Study of Protein Dynamics via Neutron Spin Echo Spectroscopy

Published on: April 13, 2022

Related Experiment Videos

Last Updated: Mar 19, 2026

Author Spotlight: Photo Switchable Protein Recruitment for Reversible Patterning in Artificial Cellular Systems

Author Spotlight: Photo Switchable Protein Recruitment for Reversible Patterning in Artificial Cellular Systems

Published on: February 23, 2024

Tracking Single Proteins in Lipid Bilayers Using Fluorescence Microscopy

Tracking Single Proteins in Lipid Bilayers Using Fluorescence Microscopy

Published on: December 12, 2025

Study of Protein Dynamics via Neutron Spin Echo Spectroscopy

Study of Protein Dynamics via Neutron Spin Echo Spectroscopy

Published on: April 13, 2022

Area of Science:

Cell Biology
Biophysics
Microscopy

Background:

Understanding molecular mechanisms in biological systems relies on visualizing protein dynamics.
Fluorescent microscopy advancements enable high-resolution, time-resolved studies of molecular behavior in vivo.
Light sheet microscopy is ideal for rapid, in vivo imaging across various specimen scales.

Purpose of the Study:

To summarize the principles and advantages of light sheet microscopy.
To highlight innovations enhancing spatio-temporal resolution in microscopy.
To provide guidelines for implementing light sheet microscopy and demonstrate its application in whole-organism protein dynamics.

Main Methods:

Review of light sheet microscopy principles and comparison with other optical imaging techniques (light microscopy, confocal microscopy).
Discussion of recent innovations improving spatio-temporal resolution.
Demonstration of light sheet microscopy applications for measuring protein dynamics in vivo.

Main Results:

Light sheet microscopy offers advantages for rapid in vivo imaging of diverse biological specimens.
Recent innovations significantly improve the spatio-temporal resolution of light sheet microscopy.
The technology effectively measures protein dynamics within a whole-organism context.

Conclusions:

Light sheet microscopy is a powerful tool for visualizing protein dynamics and understanding molecular mechanisms.
Its high resolution and in vivo capabilities make it suitable for studying complex biological systems.
This technique facilitates quantitative analysis of molecular behavior in living cells and organisms.