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A High Throughput MHC II Binding Assay for Quantitative Analysis of Peptide Epitopes
Published on: March 25, 2014
In silico cloning and B/T cell epitope prediction of triosephosphate isomerase from Echinococcus granulosus
1Department of Pathogenic Biology, Chongqing Medical University, Chongqing, 400016, China.
Abstract:
Cystic echinococcosis is a worldwide zoonosis caused by Echinococcus granulosus. Because the methods of diagnosis and treatment for cystic echinococcosis were limited, it is still necessary to screen target proteins for the development of new anti-hydatidosis vaccine. In this study, the triosephosphate isomerase gene of E. granulosus was in silico cloned. The B cell and T cell epitopes were predicted by bioinformatics methods. The cDNA sequence of EgTIM was composition of 1094 base pairs, with an open reading frame of 753 base pairs. The deduced amino acid sequences were composed of 250 amino acids. Five cross-reactive epitopes, locating on 21aa-35aa, 43aa-57aa, 94aa-107aa, 115-129aa, and 164aa-183aa, could be expected to serve as candidate epitopes in the development of vaccine against E. granulosus. These results could provide bases for gene cloning, recombinant expression, and the designation of anti-hydatidosis vaccine.
Insights
Researchers identified potential vaccine targets for cystic echinococcosis by analyzing the Echinococcus granulosus triosephosphate isomerase gene. This study predicts key epitopes for developing a new anti-hydatidosis vaccine.
Area of Science:
- Parasitology
- Vaccinology
- Bioinformatics
Background:
- Cystic echinococcosis, a global zoonosis caused by Echinococcus granulosus, faces diagnostic and treatment limitations.
- The need for novel vaccine strategies against hydatidosis is critical.
Purpose of the Study:
- To identify potential target proteins for a new anti-hydatidosis vaccine.
- To in silico clone the triosephosphate isomerase (EgTIM) gene of E. granulosus.
- To predict B cell and T cell epitopes for vaccine development.
Main Methods:
- In silico cloning of the E. granulosus triosephosphate isomerase gene.
- Bioinformatic prediction of B cell and T cell epitopes.
- Analysis of cDNA and deduced amino acid sequences.
Main Results:
- The EgTIM cDNA sequence comprises 1094 base pairs with a 753 base pair open reading frame.
- The deduced amino acid sequence consists of 250 amino acids.
- Five cross-reactive epitopes were identified at specific amino acid positions (21-35, 43-57, 94-107, 115-129, and 164-183).
Conclusions:
- The identified epitopes are potential candidates for developing a vaccine against Echinococcus granulosus.
- These findings provide a foundation for gene cloning, recombinant expression, and anti-hydatidosis vaccine design.
