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Published on: July 11, 2016
Complete Genome Sequence of Sphingopyxis macrogoltabida Strain 203N (NBRC 111659), a Polyethylene Glycol Degrader
Yoshiyuki Ohtsubo1, Shouta Nonoyama, Yuji Nagata2
1Department of Environmental Life Sciences, Graduate School of Life Sciences, Tohoku University, Sendai, Japan yohtsubo@ige.tohoku.ac.jp.
Abstract:
We determined the complete genome sequence of Sphingopyxis macrogoltabida strain 203N, a polyethylene glycol degrader. Because the PacBio assembly (285× coverage) seemed to be full of nucleotide-level mismatches, the Newbler assembly of MiSeq mate-pair and paired-end data was used for finishing and the PacBio assembly was used as a reference. The PacBio assembly carried 414 nucleotide mismatches over 5,953,153 bases of the 203N genome.
