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Related Concept Videos

Labeling DNA Probes03:31

Labeling DNA Probes

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DNA probes are fragments of DNA labeled with a reporter tag to enable their detection or purification. The resulting labeled DNA probes can then hybridize to target nucleic acid sequences through complementary base-pairing, and may be used to recover or identify these regions.
Radioisotopes, fluorophores, or small molecule binding partners like biotin or digoxigenin, are the most widely used reporter tags for labeling DNA probes. These labels can be attached to the probe DNA molecule via...
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Design and Development of Aptamer&#8211;Gold Nanoparticle Based Colorimetric Assays for In-the-field Applications
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DNA module platform for developing colorimetric aptamer sensors.

Yasuyuki Tomita1, Yuji Morita1, Hiroaki Suga2

  • 1Central Laboratories for Key Technologies, KIRIN Company, Ltd, Yokohama, Japan.

Biotechniques
|June 12, 2016
PubMed
Summary

This study introduces a DNA module platform for creating aptamer sensors. This innovative system simplifies sensor development using microarrays and computational predictions, enabling rapid detection of targets like arginine and patulin.

Keywords:
DNA module platformaptamer microarrayaptamer-based sensorin silico design

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Area of Science:

  • Biotechnology
  • Molecular Biology
  • Biosensor Development

Background:

  • Aptamer-based sensors offer high specificity but often require complex development.
  • Integrating computational tools can streamline aptamer sensor design and screening.

Purpose of the Study:

  • To present a versatile DNA module platform for constructing simple aptamer sensors.
  • To demonstrate the platform's utility in creating sensors for specific targets and discovering new aptamers.

Main Methods:

  • Development of a DNA module platform incorporating aptamer, joint module, terminal stem, and DNAzyme reporter.
  • Utilizing in silico secondary structure prediction for sequence design and microarray customization.
  • Employing a DNAzyme reporter for signal transduction based on aptamer-target binding-induced conformational changes.

Main Results:

  • Successfully created concentration-dependent sensors for AMP and arginine by modular assembly.
  • Identified a novel aptamer against patulin using the platform and a microarray format with over 10,000 sequences.
  • Demonstrated the platform's adaptability for both known and novel aptamer sensor development.

Conclusions:

  • The DNA module platform provides a flexible and efficient approach for aptamer sensor development.
  • This system facilitates the rapid creation of custom biosensors and the discovery of new aptamers.
  • The combination of modular design, microarray format, and in silico prediction accelerates biosensor innovation.