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Biological Compatibility Profile on Biomaterials for Bone Regeneration
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Surface biofunctionalization of β-TCP blocks using aptamer 74 for bone tissue engineering.

N Ardjomandi1, J Huth1, D R Stamov2

  • 1Department of Oral and Maxillofacial Surgery, University Hospital Tübingen, Germany.

Materials Science & Engineering. C, Materials for Biological Applications
|June 12, 2016
PubMed
Summary

Aptamer 74 functionalized beta-tricalcium phosphate (β-TCP) enhances jaw periosteal cell (JPC) adhesion and mineralization for bone regeneration. This biomaterial shows promise for oral and maxillofacial surgery applications.

Keywords:
AFM-based force spectroscopyAptamerTissue engineeringsurface biofunctionalizationβ-tricalcium phosphate (β-TCP)

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Area of Science:

  • Biomaterials Science
  • Tissue Engineering
  • Regenerative Medicine

Background:

  • Bone regeneration in oral and maxillofacial surgery requires effective strategies to recruit osteogenic progenitor cells.
  • Jaw periosteal cells (JPCs) are crucial for bone formation, and aptamer 74 has shown affinity for osteogenically induced JPCs.

Purpose of the Study:

  • To biofunctionalize beta-tricalcium phosphate (β-TCP) surfaces with aptamer 74 for enhanced osteogenic cell interactions.
  • To evaluate the effect of aptamer 74 immobilization on JPC adhesion, proliferation, and mineralization on β-TCP constructs.

Main Methods:

  • Surface functionalization of β-TCP blocks with aptamer 74.
  • Atomic force microscopy (AFM) to assess aptamer binding activity.
  • QuantiFluor ssDNA assay and amino acid analysis to determine immobilization efficiency.
  • In vitro cell culture assays to evaluate JPC proliferation, adhesion, and mineralization.

Main Results:

  • Aptamer 74 demonstrated increased binding to osteogenically induced JPCs.
  • No significant difference in cell proliferation was observed between aptamer-functionalized and unmodified surfaces.
  • Significantly higher JPC mineralization occurred on 2D aptamer-functionalized surfaces.
  • Enhanced JPC adhesion was detected on 3D aptamer-functionalized β-TCP constructs.

Conclusions:

  • A biocompatible β-TCP matrix functionalized with aptamer 74 enhances JPC adhesion on 3D constructs and mineralization on 2D surfaces.
  • This functionalized β-TCP shows potential for improving bone regeneration strategies in oral and maxillofacial applications.
  • Further research is needed to optimize aptamer immobilization and JPC mineralization within 3D constructs.