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Down-regulation of nestin in mesenchymal stem cells derived from peripheral blood through blocking bone morphogenesis

Zahra Fazeli1, Mir Davood Omrani2,3, Sayyed Mohammad Hossein Ghaderian4,5

  • 1Department of Medical Genetics, Faculty of Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran.

Journal of Cell Communication and Signaling
|June 12, 2016
PubMed

Insights

Blocking Bone Morphogenesis Pathway (BMP) signaling in mesenchymal stem cells reduced Nestin expression. This suggests BMP signaling regulates Nestin in these cells.

Area of Science:

  • Stem cell biology
  • Molecular signaling pathways

Background:

  • Mesenchymal stem cells (MSCs) differentiate into various cell types.
  • Bone Morphogenesis Pathway (BMP) signaling is crucial for osteogenic and adipogenic differentiation of MSCs.
  • Nestin is a neural stem cell marker.

Purpose of the Study:

  • To investigate the effect of blocking BMP signaling on Nestin expression in peripheral blood-derived MSCs (PB-MSCs).

Main Methods:

  • Isolation of MSCs from peripheral blood using plastic adherence and flow cytometry.
  • Treatment of MSCs with Noggin, a BMP signaling antagonist, for 8 days.
  • Real-time PCR analysis to quantify Nestin expression.

Main Results:

  • BMP signaling attenuation led to a significant decrease in Nestin expression in PB-MSCs.
  • Noggin treatment effectively blocked BMP signaling in the studied cells.

Conclusions:

  • Bone Morphogenesis Pathway (BMP) signaling appears to regulate Nestin expression in mesenchymal stem cells.
  • This finding contributes to understanding stem cell differentiation and marker expression.
Keywords:
BMP signalingMesenchymal stem cells (MSCs)NestinNoggin

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