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Immunogold Electron Microscopy

Immunogold Electron Microscopy

Immunoelectron microscopy utilizes immunogold labeling of endogenous proteins with specific antibodies to detect and localize these proteins in cells and tissues. The procedure provides insights into the distribution and quantification of protein under different stimulation conditions offering clues about their functions. Conjugating highly electron-dense gold particles with primary or secondary antibodies allow antigen detection on and within cells, with high resolution and specificity.

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Related Experiment Video

Updated: Mar 19, 2026

Revealing Neural Circuit Topography in Multi-Color

Revealing Neural Circuit Topography in Multi-Color

Published on: November 14, 2011

Enhancing Fluorogold-based neural tract tracing.

S E Mondello¹, S C Jefferson², W A O'Steen³

¹Department of Rehabilitation Medicine, University of Washington, Seattle, WA 98195, United States.

Journal of Neuroscience Methods

|June 12, 2016

Summary

This summary is machine-generated.

This study introduces a method using Triton™ to enhance Fluorogold (FG) neural tracing. The new technique reduces FG toxicity and tracing time, minimizing motor deficits and improving detection sensitivity.

Keywords:

Detergent Fluorogold Immunohistochemistry Neurotoxic Retrograde tracing Triton

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Last Updated: Mar 19, 2026

Revealing Neural Circuit Topography in Multi-Color

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Post-embedding Immunogold Labeling of Synaptic Proteins in Hippocampal Slice Cultures

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Published on: April 3, 2013

Area of Science:

Neuroscience
Molecular Biology
Biochemistry

Background:

Fluorogold (FG) is a widely used retrograde tracer for nervous system pathways.
However, FG exhibits neurotoxicity, causing injection site damage and motor deficits.

Purpose of the Study:

To describe a novel method for enhancing FG uptake.
To present a procedure for minimizing FG-related tissue damage and improving quantification.

Main Methods:

Utilized Triton™ to enhance FG uptake and transport.
Employed FG antiserum with an ABC chromogen reaction for improved fluorescence detection.
Investigated long-distance tracing from thoracic spinal cord to motor cortex.

Main Results:

Triton™ enhanced FG uptake and reduced required FG amounts and tracing time by over 4-fold for distances greater than 10 inches.
Reduced FG concentrations and volumes minimized tissue damage and motor deficits.
FG antiserum and ABC chromogen reaction provided stable, detectable fluorescence.

Conclusions:

Triton™ enhances FG neural tracing by improving uptake and reducing toxicity and tracing time.
The method effectively minimizes FG-induced tissue damage and motor deficits.
Improved detection methods ensure reliable quantification despite low FG concentrations.