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Published on: October 23, 2011
Identification of Lactobacillus brevis using a species-specific AFLP-derived marker
Vincenzina Fusco1, Grazia Marina Quero1, Daniele Chieffi1
1National Research Council of Italy, Institute of Sciences of Food Production (CNR-ISPA), Bari, Italy.
Abstract:
A simple and specific method for the rapid detection and identification of Lactobacillus brevis was developed. A fAFLP (Fluorescent Amplified Fragment Length Polymorphisms) marker for L. brevis was used to design oligonucleotide primers for a species-specific PCR assay, targeting a 125bp fragment of the gene encoding the aldo/keto reductase of the diketogulonate-reductase family of L. brevis. This assay resulted in 100% inclusivity and exclusivity of assignment of strains to the species L. brevis. The analytical specificity of this assay was successfully tested to identify L. brevis isolates from sourdoughs.
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