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Updated: Mar 19, 2026

A Sensitive Visual Method for the Detection of Hydrogen Sulfide Producing Bacteria
Published on: June 27, 2022
A novel fluorescent probe for rapid and sensitive detection of hydrogen sulfide in living cells
Jian Pan1, Junchao Xu1, Youlai Zhang2
1Department of Chemistry and Material Sciences, Hubei Engineering University, Hubei, Xiaogan 432000, PR China; School of Chemistry & Chemical Engineering, Tianjin University of Technology, Tianjin 300384, PR China.
Abstract:
A novel fluorescent probe for H2S was developed based on a far-red emitting indole-BODIPY, which was decorated with morpholine and 2,4-dinitrobenzenesulfonyl (DNBS) group. This probe showed rapid response (t1/2=3min), high selectivity and sensitivity for H2S with significant colorimetric and fluorescence OFF-ON signals, which was triggered by cleavage of 2,4-dinitrobenzenesulfonyl group. This probe could quantitatively detect the concentrations of H2S ranging from 0 to 60μM, and the detection of limit was found to be as low as 26nM. Cell imaging results indicated that the probe could detect and visualize H2S in the living cells.
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