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Updated: Mar 19, 2026

Homogeneous Glycoconjugate Produced by Combined Unnatural Amino Acid Incorporation and Click-Chemistry for Vaccine Purposes
Published on: December 19, 2020
Chemically synthesized glycoconjugates on proteins: effects of multivalency and glycoform in vivo
1Biofunctional Synthetic Chemistry Laboratory, RIKEN, 2-1 Hirosawa, Wako-shi, Saitama 351-0198, Japan. kotzenori@riken.jp and Biofunctional Chemistry Laboratory, A. Butlerov Institute of Chemistry, Kazan Federal University, 18 Kremlyovskaya street, Kazan 420008, Russia and JST PRESTO, 2-1 Hirosawa, Wako-shi, Saitama 351-0198, Japan.
Abstract:
The biodistributions and in vivo kinetics of chemically prepared glycoconjugates on proteins are reviewed. Chemical methods can be used to conjugate various mono- and oligosaccharides onto a protein surface. The kinetics and organ-specific accumulation profiles of these glycoconjugates, which are introduced through intravenous injections, have been analyzed using conventional dissection studies as well as non-invasive methods such as single photon emission computed tomography (SPECT), positron emission tomography (PET), and fluorescence imaging. The results suggest that glycan-dependent protein distribution kinetics may be useful for pharmacological and diagnostic applications.
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