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Use of Human Perivascular Stem Cells for Bone Regeneration
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An Optimized Injectable Hydrogel Scaffold Supports Human Dental Pulp Stem Cell Viability and Spreading.

T D Jones1, A Kefi1, S Sun1

  • 1Bioengineering, University of Illinois at Chicago, Chicago, IL 60612-7212, USA.

Advances in Medicine
|June 14, 2016
PubMed
Summary

Injectable hydrogels made from polyethylene glycol diacrylate (PEGDA), hyaluronan (HA), and gelatin (Gn) support human dental pulp stem cell (hDPSC) viability and spreading. Optimizing HA:Gn ratios and adding fibronectin (Fn) further enhances cell behavior in these biomaterials.

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Area of Science:

  • Biomaterials Science
  • Stem Cell Biology
  • Tissue Engineering

Background:

  • Injectable hydrogels are promising for regenerative medicine.
  • HyStem-C™ (PEGDA-HA-Gn) is a tunable hydrogel for cell encapsulation.
  • Optimizing hydrogel properties is crucial for clinical applications.

Purpose of the Study:

  • To determine the optimal concentration of PEGSSDA for injectable hydrogels.
  • To evaluate the impact of HA:Gn ratios on hDPSC viability and spreading.
  • To investigate the effect of fibronectin (Fn) on hDPSC behavior within the hydrogel.

Main Methods:

  • Varied PEGSSDA concentration (0.5-8.0%) to assess gelation time and injectability.
  • Encapsulated hDPSCs in PEGSSDA-HA-Gn hydrogels with varying HA:Gn ratios (100:0 to 25:75).
  • Incorporated fibronectin (Fn) at different concentrations (0.1, 1.0, 10.0 μg/mL) to study its effect on cell proliferation and spreading.

Main Results:

  • Hydrogel gelation time decreased with increasing PEGSSDA concentration.
  • PEGSSDA-HA-Gn hydrogels demonstrated biocompatibility with hDPSCs.
  • Higher HA:Gn ratios (e.g., 75:25) enhanced hDPSC viability over 14 days.
  • Fibronectin significantly increased hDPSC proliferation at 1.0 and 10.0 μg/mL and spreading at 0.1 μg/mL.

Conclusions:

  • Polyethylene glycol-based injectable hydrogels effectively maintain hDPSC viability.
  • Hydrogel composition, particularly HA:Gn ratio, influences cell viability.
  • Extracellular matrix proteins like fibronectin significantly promote hDPSC proliferation and spreading within the hydrogel, highlighting their importance for cell behavior.