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An Optimized Injectable Hydrogel Scaffold Supports Human Dental Pulp Stem Cell Viability and Spreading
1Bioengineering, University of Illinois at Chicago, Chicago, IL 60612-7212, USA.
Advances in Medicine
|June 14, 2016
Summary
Injectable hydrogels made from polyethylene glycol diacrylate (PEGDA), hyaluronan (HA), and gelatin (Gn) support human dental pulp stem cell (hDPSC) viability and spreading. Optimizing HA:Gn ratios and adding fibronectin (Fn) further enhances cell behavior in these biomaterials.
Area of Science:
- Biomaterials Science
- Stem Cell Biology
- Tissue Engineering
Background:
- Injectable hydrogels are promising for regenerative medicine.
- HyStem-C™ (PEGDA-HA-Gn) is a tunable hydrogel for cell encapsulation.
- Optimizing hydrogel properties is crucial for clinical applications.
Purpose of the Study:
- To determine the optimal concentration of PEGSSDA for injectable hydrogels.
- To evaluate the impact of HA:Gn ratios on hDPSC viability and spreading.
- To investigate the effect of fibronectin (Fn) on hDPSC behavior within the hydrogel.
Main Methods:
- Varied PEGSSDA concentration (0.5-8.0%) to assess gelation time and injectability.
- Encapsulated hDPSCs in PEGSSDA-HA-Gn hydrogels with varying HA:Gn ratios (100:0 to 25:75).
- Incorporated fibronectin (Fn) at different concentrations (0.1, 1.0, 10.0 μg/mL) to study its effect on cell proliferation and spreading.
Main Results:
- Hydrogel gelation time decreased with increasing PEGSSDA concentration.
- PEGSSDA-HA-Gn hydrogels demonstrated biocompatibility with hDPSCs.
- Higher HA:Gn ratios (e.g., 75:25) enhanced hDPSC viability over 14 days.
- Fibronectin significantly increased hDPSC proliferation at 1.0 and 10.0 μg/mL and spreading at 0.1 μg/mL.
Conclusions:
- Polyethylene glycol-based injectable hydrogels effectively maintain hDPSC viability.
- Hydrogel composition, particularly HA:Gn ratio, influences cell viability.
- Extracellular matrix proteins like fibronectin significantly promote hDPSC proliferation and spreading within the hydrogel, highlighting their importance for cell behavior.
