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Developing a Salivary Antibody Multiplex Immunoassay to Measure Human Exposure to Environmental Pathogens
Published on: September 12, 2016
Host factors associated with serologic inflammatory markers assessed using multiplex assays.
Heather S McKay1, Jay H Bream2, Joseph B Margolick2
1Department of Epidemiology, Johns Hopkins Bloomberg School of Public Health, Baltimore, MD, USA.
Chronic systemic inflammation is linked to health issues. This study found hypertension, obesity, and diabetes are associated with higher inflammation biomarkers in men.
Area of Science:
- Immunology
- Epidemiology
- Biomarker Research
Background:
- Chronic systemic inflammation is a known contributor to adverse health outcomes.
- Understanding the impact of risk factors on inflammation biomarkers is crucial for public health.
- Limited data exists on how fixed and modifiable factors influence a wide range of inflammation biomarkers.
Purpose of the Study:
- To investigate the associations between fixed and modifiable risk factors and serum concentrations of 23 inflammation biomarkers.
- To identify specific biomarkers that correlate with conditions like hypertension, obesity, and diabetes.
- To inform epidemiological studies and potential therapeutic targets for inflammation-related diseases.
Main Methods:
- Longitudinal analysis of serum samples from 250 HIV-uninfected men in the Multicenter AIDS Cohort Study (1984-2009).
- Measurement of 23 biomarkers including C-reactive protein (CRP), cytokines, and soluble immune receptors using multiplexed immunometric assays.
- Generalized gamma regression applied to identify statistically significant associations between risk factors and biomarker concentrations, adjusting for covariates.
Main Results:
- Higher concentrations of specific biomarkers were significantly associated with hypertension, obesity, hepatitis C infection, stimulant use, and diabetes (P<0.002).
- Lower concentrations of certain biomarkers were linked to hypercholesterolemia.
- Associations were adjusted for age, race, and education, and considered for multiple biomarker analysis.
Conclusions:
- Specific risk factors, including hypertension, obesity, and diabetes, are significantly associated with altered levels of inflammation biomarkers.
- These findings highlight the importance of considering these associations in epidemiological research involving inflammation markers.
- Identified biomarker-risk factor links may offer potential targets for disease prevention and treatment strategies.
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