Related Experiment Videos
Analytical determination of methylated histidine in proteins: actin methylation
M Raghavan1, C K Smith, C E Schutt
1Department of Chemistry, Princeton University, New Jersey 08544.
Analytical Biochemistry
|April 1, 1989
Abstract:
The methylation of histidine in actin from various muscle and nonmuscle sources has been studied by formation of phenylthiocarbamyl derivatives and subsequent reverse-phase high-pressure liquid chromatographic separation and analysis of actin hydrolyzates. All the actin species examined were found to contain 3-methylhistidine. This method has also been used in assays for the enzyme(s) responsible for methylation of rabbit skeletal muscle actin and to investigate the formation of other methylated residues in vitro. 3-Methyl-histidine is the major methylation product in this in vitro reaction.