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[Biodistribution and Postmortem Redistribution of Emamectin Benzoate in Intoxicated Mice]
Objective:
To investigate the lethal blood level, the target organs and tissues, the toxicant storage depots and the postmortem redistribution in mice died of emamectin benzoate poisoning.
Methods:
The mice model of emamectin benzoate poisoning was established via intragastric injection. The main poisoning symptoms and the clinical death times of mice were observed and recorded dynamically in the acute poisoning group as well as the sub-acute poisoning death group. The pathological and histomorphological changes of organs and tissues were observed after poisoning death. The biodistribution and postmortem redistribution of emamectin benzoate in the organs and tissues of mice were assayed by the enzyme-linked immunosorbent assay (ELISA) at 0h, 24h, 48h and 72h after death. The lethal blood concentrations and the concentrations of emamectin benzoate were detected by high performance liquid chromatography (HPLC) at different time points after death.
Results:
The symptoms of nervous and respiratory system were observed within 15-30 min after intragastric injection. The average time of death was (45.8 ± 7.9) min in the acute poisoning group and (8.0 ± 1.4) d in the sub-acute poisoning group, respectively. The range of acute lethal blood level was 447.164 0-524.463 5 mg/L. The pathological changes of the organs and tissues were observed via light microscope and immunofluorescence microscope. The changes of emamectin benzoate content in the blood, heart, liver, spleen, lung, kidney and brain of poisoning mice showed regularity within 72 h after death (P < 0.05).
Conclusion:
The target organs of emamectin benzoate poisoning include heart, liver, kidney, lung, brain and contact position (stomach). The toxicant storage depots are kidney and liver. There is emamectin benzoate postmortem redistribution in mice.
Insights
Emamectin benzoate poisoning in mice affects the nervous and respiratory systems, with lethal blood levels ranging from 447-525 mg/L. Key target organs include the heart, liver, and kidneys, with evidence of postmortem redistribution.
Area of Science:
- Toxicology
- Pharmacology
- Veterinary Medicine
Background:
- Emamectin benzoate is a widely used insecticide.
- Understanding its toxicokinetics and target organs is crucial for risk assessment and management.
Purpose of the Study:
- To determine the lethal blood levels of emamectin benzoate in mice.
- To identify target organs, toxicant storage sites, and postmortem redistribution patterns.
Main Methods:
- Establishing a mouse model of emamectin benzoate poisoning via intragastric administration.
- Monitoring poisoning symptoms and survival times.
- Analyzing pathological changes using microscopy.
- Quantifying emamectin benzoate levels in blood and tissues using ELISA and HPLC at various postmortem intervals.
Main Results:
- Acute poisoning symptoms appeared within 15-30 minutes, with average death times of ~46 minutes (acute) and ~8 days (sub-acute).
- Acute lethal blood levels ranged from 447.16 to 524.46 mg/L.
- Significant changes in emamectin benzoate concentrations were observed in blood, heart, liver, spleen, lungs, kidneys, and brain within 72 hours postmortem.
Conclusions:
- The primary target organs for emamectin benzoate toxicity include the heart, liver, kidneys, lungs, brain, and stomach.
- The kidneys and liver serve as primary toxicant storage depots.
- Emamectin benzoate exhibits postmortem redistribution in mice.
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