[Construction of a 15-plex Rapid STR Multiplex Amplification System]

Area of Science:

• Forensic science
• Molecular biology
• Genetics

Background:

• Short tandem repeat (STR) analysis is crucial in forensic science.
• Optimizing STR multiplex amplification is essential for increasing sample throughput and reducing analysis time.

Purpose of the Study:

• To establish a rapid 15-plex STR multiplex amplification system.
• To optimize conditions for efficient and accurate STR profiling.

Main Methods:

• Selected 14 autosomal and 1 sex chromosome STR loci.
• Utilized FastStart Taq DNA polymerase and standard DNA samples for optimization.
• Investigated various amplification conditions, polymerase volumes, inter-locus balance, buffers, reaction volumes, and additives.

Main Results:

• Achieved a complete and balanced 15-STR profile from standard genomic DNA in 32 minutes using a 10 μL rapid PCR system.
• No allele drop-out or non-specific amplicons were observed.
• Identified effective reactive additives including glycerinum, gelatin, and ammonium sulfate.

Conclusions:

• The developed 15-plex rapid STR multiplex amplification system effectively reduces reaction time.
• This system enhances sample throughput, making it valuable for forensic applications.