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Updated: Mar 19, 2026

Anaerobic Protein Purification and Kinetic Analysis via Oxygen Electrode for Studying DesB Dioxygenase Activity and Inhibition
Published on: October 3, 2018
An iron-oxygen intermediate formed during the catalytic cycle of cysteine dioxygenase
E P Tchesnokov1, A S Faponle2, C G Davies1
1Department of Chemistry & MacDiarmid Institute for Advanced Materials and Nanotechnology University of Otago, PO Box 56, Dunedin 9054, New Zealand. guy.jameson@otago.ac.nz.
Abstract:
Cysteine dioxygenase is a key enzyme in the breakdown of cysteine, but its mechanism remains controversial. A combination of spectroscopic and computational studies provides the first evidence of a short-lived intermediate in the catalytic cycle. The intermediate decays within 20 ms and has absorption maxima at 500 and 640 nm.
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