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Identification of Circular RNAs using RNA Sequencing
Published on: November 14, 2019
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Circular RNA expression in cutaneous squamous cell carcinoma
Michael Sand1, Falk G Bechara2, Thilo Gambichler2
1Dermatologic Surgery Unit, Department of Dermatology, Venereology and Allergology, Ruhr-University Bochum 44791 Bochum, Germany; Department of Plastic Surgery, St. Josef Hospital, Catholic Clinics of the Ruhr Peninsula, 45257 Essen, Germany.
Journal of Dermatological Science
|June 15, 2016
Summary
Circular RNAs (circRNAs) are differentially expressed in cutaneous squamous cell carcinoma (cSCC). These circRNAs impact tumor formation by interacting with microRNAs (miRNAs) involved in cSCC development.
Area of Science:
- Molecular Biology
- Epigenetics
- Oncology
Background:
- Circular RNAs (circRNAs) are abundant, stable, and conserved non-coding RNAs.
- circRNAs regulate cellular networks and epigenetics by sequestering microRNAs (miRNAs).
Purpose of the Study:
- To investigate circRNA expression in cutaneous squamous cell carcinoma (cSCC).
Main Methods:
- Microarray analysis of circRNA expression in cSCC and control skin biopsies.
- Validation of microarray data using quantitative real-time reverse transcription polymerase chain reaction.
- Bioinformatic analysis to identify miRNA response elements (MREs) in circRNAs interacting with relevant miRNAs.
Main Results:
- Identified 322 differentially expressed circRNAs (143 up-regulated, 179 down-regulated) in cSCC.
- Found 1603 MREs within differentially expressed circRNAs.
- Identified complementary MRE sequences in 23 cSCC-relevant miRNAs.
Conclusions:
- circRNAs are differentially expressed in cSCC.
- circRNAs play a role in cSCC formation by interfering with relevant miRNAs.
- circRNA-miRNA interactions contribute to epigenetic control in cSCC.
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