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Identification of glucagon-like peptide-1(7-36) amide in rat brain
S Yoshimoto1, M Hirota, C Ohboshi
1Department of Laboratory Medicine, School of Medicine, University of Tokushima, Japan.
Annals of Clinical Biochemistry
|March 1, 1989
Abstract:
Acid-urea extract of rat brain was examined by glucagon-like peptide-1 (GLP-1) specific radioimmunoassay. A single peak was observed which co-eluted with GLP-1(7-36)amide on gel filtration and anion exchange chromatography. In contrast, GLP-1(1-37) was not detected under our experimental conditions. The fact that GLP-1 (7-36)amide, but not GLP-1(1-37), was present in rat brain suggests that preproglucagon was processed in the brain in the same manner as in the intestine and not as in the pancreas.