Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Real Time RT-PCR02:57

Real Time RT-PCR

66.4K
Real-time reverse transcription-polymerase chain reaction, or Real-time RT-PCR, is an analytical tool used to determine the expression level of target genes. The method involves converting mRNA to complementary DNA with the help of an enzyme known as reverse transcriptase, followed by the PCR amplification of the cDNA. These two processes can be performed simultaneously in a single tube or separately as a two-step reaction.
The real-time quantification of the number of amplified products is...
66.4K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Unsupervised Machine-Learning-Based Endotype Discovery Using Iterative Resampling in Dupilumab-Treated Patients.

International journal of molecular sciences·2026
Same author

Postbiotics and Nicotinamide Utilize Distinct Mechanisms to Improve Skin Barrier Integrity, Inflammation, and Keratinocyte Differentiation.

Allergy·2026
Same author

Monosodium Glutamate Induces Cellular Stress, Endoplasmic Reticulum Stress, Mitochondrial Dysfunction, and Cell Death in Intestinal Epithelial Cells.

Allergy·2025
Same author

Type 2 immunity in allergic diseases.

Cellular & molecular immunology·2025
Same author

<i>TSLP</i> and <i>TSLPR</i> Expression Levels in Peripheral Blood as Potential Biomarkers in Patients with Chronic Rhinosinusitis with Nasal Polyps.

International journal of molecular sciences·2025
Same author

Epithelial barrier dysfunction and associated diseases in companion animals: Differences and similarities between humans and animals and research needs.

Allergy·2024

Related Experiment Video

Updated: Mar 19, 2026

Characterization of Inflammatory Responses During Intranasal Colonization with Streptococcus pneumoniae
09:12

Characterization of Inflammatory Responses During Intranasal Colonization with Streptococcus pneumoniae

Published on: January 17, 2014

13.2K

Real-Time PCR for Gene Expression Quantification in Asthma.

Ignacio San Segundo-Val1,2, Virginia García-Solaesa3,4, Asunción García-Sánchez5

  • 1Department of Clinical Biochemistry, University Hospital of Salamanca, Salamanca, Spain. i.sansegundo.val@gmail.com.

Methods in Molecular Biology (Clifton, N.J.)
|June 15, 2016
PubMed
Summary

Quantitative real-time PCR (qPCR) using SYBR Green dye is a key method for analyzing gene expression. This protocol details RNA extraction, cDNA synthesis, and data analysis for studying asthma mechanisms.

Keywords:
AsthmaExpression analysisRT-qPCR (reverse transcription-qPCR)cDNAmRNAqPCR

More Related Videos

Single-cell Gene Expression Profiling Using FACS and qPCR with Internal Standards
10:50

Single-cell Gene Expression Profiling Using FACS and qPCR with Internal Standards

Published on: February 25, 2017

17.4K
Author Spotlight: Exploring the Role of Inflammation in the Co-occurrence of Primary Sjogren's Syndrome and Lung Adenocarcinoma
10:21

Author Spotlight: Exploring the Role of Inflammation in the Co-occurrence of Primary Sjogren's Syndrome and Lung Adenocarcinoma

Published on: September 20, 2024

945

Related Experiment Videos

Last Updated: Mar 19, 2026

Characterization of Inflammatory Responses During Intranasal Colonization with Streptococcus pneumoniae
09:12

Characterization of Inflammatory Responses During Intranasal Colonization with Streptococcus pneumoniae

Published on: January 17, 2014

13.2K
Single-cell Gene Expression Profiling Using FACS and qPCR with Internal Standards
10:50

Single-cell Gene Expression Profiling Using FACS and qPCR with Internal Standards

Published on: February 25, 2017

17.4K
Author Spotlight: Exploring the Role of Inflammation in the Co-occurrence of Primary Sjogren's Syndrome and Lung Adenocarcinoma
10:21

Author Spotlight: Exploring the Role of Inflammation in the Co-occurrence of Primary Sjogren's Syndrome and Lung Adenocarcinoma

Published on: September 20, 2024

945

Area of Science:

  • Molecular Biology
  • Genetics
  • Biochemistry

Background:

  • Quantitative real-time PCR (qPCR) is the standard for gene expression studies.
  • Understanding gene expression is crucial for studying diseases like asthma.

Purpose of the Study:

  • To describe a protocol for quantifying gene expression using SYBR Green dye.
  • To provide a method for analyzing gene expression mechanisms in asthma.

Main Methods:

  • RNA extraction from biological samples.
  • Reverse transcription of RNA to complementary DNA (cDNA).
  • Real-time PCR quantification using SYBR Green fluorescent dye.

Main Results:

  • The protocol enables accurate quantification of gene expression levels.
  • SYBR Green dye provides a reliable method for RNA quantification.
  • The described workflow facilitates gene expression analysis in asthma research.

Conclusions:

  • This protocol offers a standardized approach for gene expression analysis via qPCR.
  • The method is valuable for elucidating gene expression mechanisms in asthma.
  • SYBR Green-based qPCR is an effective tool for molecular diagnostics and research.