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Related Concept Videos

RNA-seq03:21

RNA-seq

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RNA sequencing, or RNA-Seq, is a high-throughput sequencing technology used to study the transcriptome of a cell. Transcriptomics helps to interpret the functional elements of a genome and identify the molecular constituents of an organism. Additionally, it also helps in understanding the development of an organism and the occurrence of diseases. 
Before the discovery of RNA-seq, microarray-based methods and Sanger sequencing were used for transcriptome analysis. However, while...
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Ribosome Profiling02:24

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Ribosome profiling or ribo-sequencing is a deep sequencing technique that produces a snapshot of active translation in a cell. It selectively sequences the mRNAs protected by ribosomes to get an insight into a cell’s translation landscape at any given point in time.
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Related Experiment Video

Updated: Mar 19, 2026

Isolation and Characterization of RNA-Containing Exosomes
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Isolation and Characterization of RNA-Containing Exosomes

Published on: January 9, 2012

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Protocols for Exosome Isolation and RNA Profiling.

Fernando Marqués-García1,2, María Isidoro-García3,4,5

  • 1Department of Clinical Biochemistry, University Hospital of Salamanca, Paseo de San Vicente 58, Salamanca, 37007, Spain. f.marg@hotmail.es.

Methods in Molecular Biology (Clifton, N.J.)
|June 15, 2016
PubMed
Summary
This summary is machine-generated.

This chapter reviews methods for isolating exosomes and purifying their RNA cargo. Exosomes, small vesicles carrying RNA, show significant clinical potential for molecular diagnostics and therapeutics.

Keywords:
BiomarkersExosomeExtracellular vesiclesIsolationPrecipitationRNA profilingUltracentrifugation

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Area of Science:

  • Cell biology
  • Molecular biology
  • Biochemistry

Background:

  • Exosomes are small extracellular vesicles originating from endosomes.
  • They contain characteristic molecular compositions in their membranes and cargo.
  • Exosomes are found in cell culture media and in vivo body fluids, produced by most cell types.

Purpose of the Study:

  • To review methodologies for exosome isolation.
  • To describe techniques for purifying RNA from exosomes.
  • To highlight the clinical potential of exosomal RNA.

Main Methods:

  • Exosome isolation techniques.
  • RNA extraction protocols from extracellular vesicles.
  • Quality control measures for isolated exosomes and RNA.

Main Results:

  • Detailed review of established and emerging methods for exosome isolation.
  • Comprehensive overview of RNA purification strategies from exosomes.
  • Discussion of RNA integrity and yield considerations.

Conclusions:

  • Effective isolation and purification are crucial for studying exosomal RNA.
  • Standardized methodologies are needed to advance the clinical applications of exosomes.
  • Exosomal RNA represents a promising biomarker and therapeutic avenue.