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[Production effect comparison of SEPP and GPx between HepG2 and Hela cells with different selenocompounds]
Objective:
To compare the effect of several selenocompounds on the productions of SEPP and GPx in HepG2 and Hela cells.
Methods:
The cultured HepG2 and Hela cells were divided into the control, Na2SeO3, SeMet and MeSeCys groups. After adding the selected selenocompounds (with the respective concentration 0.01 and 0.1 μmol/L), the experimental groups were then incubated for 48 h and 72 h. Finally, the cell culture supernatants and homogenates were collected for the SEPP and GPx concentrations detection by a double-antibody sandwich enyme-linked immuno-sorbent-assay (ELISA).
Results:
The SEPP and GPx concentrations in Hela cells treated with 0.1 μmol/L SeMet and MeSeCys were significantly higher than that in the control group (P < 0.05). The SEPP and GPx concentrations in HepG2 cell treated with 0.1 μmol/L selenocompounds were significantly higher than that in Hela cells (P < 0.05).
Conclusion:
HepG2 cells are more beneficial to the production of selenoproteins than Hela cells.
