Validation of reference genes for RT-qPCR analysis in Herbaspirillum seropedicae
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Published on: March 17, 2016
Daniella Duarte Villarinho Pessoa1, Marcia Soares Vidal2, José Ivo Baldani2
1Universidade Federal do Rio de Janeiro - UFRJ, Programa de Pós-Graduação em Biotecnologia Vegetal, Centro de Ciências da Saúde - CCS, Campus Ilha do Fundão, Av. Carlos Chagas Filho, 373 - Cidade Universitária, CEP: 21941-590 Rio de Janeiro, RJ, Brazil; Centro Nacional de Pesquisa de Agrobiologia, CNPAB, Embrapa, Laboratório de Genética e Bioquímica, Embrapa Agrobiologia, BR 465, Km 7, s/n, Pavilhão Johanna Döbereiner, Bairro Ecologia, CEP: 23890-000 Seropédica, RJ, Brazil.
Abstract:
The RT-qPCR technique needs a validated set of reference genes for ensuring the consistency of the results from the gene expression. Expression stabilities for 9 genes from Herbaspirillum seropedicae, strain HRC54, grown with different carbon sources were calculated using geNorm and NormFinder, and the gene rpoA showed the best stability values.

