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Fluorescence-Activated Cell Sorting for the Isolation of Scleractinian Cell Populations
Published on: May 31, 2020
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Flow cytometric enumeration of bacterial in the coral surface mucus layer
Yvan Bettarel1, Mai Chi Thanh2, Got Patrice1
1UMR MARBEC (IRD, CNRS, UM, IFREMER) Marine Biodiversity, Exploitation and Conservation, Montpellier, France.
Journal of Microbiological Methods
|June 16, 2016
Abstract:
The direct counts of bacteria inhabiting coral mucus were performed by flow cytometry testing four fluorescent dyes (SYBR®Green I, HCS, TOPRO®3, SYTO®62) with three different scleractinian species. Results obtained with SYTO62 were the most reliable based on the comparison with standardized epifluorescence counts and the resolution of cytograms.
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