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Template for 3D Printing a Low-Temperature Plasma Probe.

Sandra Martínez-Jarquín1, Abigail Moreno-Pedraza1, Héctor Guillén-Alonso1

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Analytical Chemistry
|June 16, 2016
PubMed
Summary

Researchers developed a reproducible low-temperature plasma (LTP) probe using commercial and 3D-printed parts for ambient mass spectrometry. This accessible device facilitates solvent-free molecular detection and imaging, advancing analytical chemistry applications.

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Area of Science:

  • Analytical Chemistry
  • Materials Science

Background:

  • Low-temperature plasma (LTP) ionization is an emerging technique for ambient mass spectrometry (MS), offering solvent-free direct detection and mass spectrometry imaging (MSI).
  • Existing LTP technologies face adoption barriers due to a lack of commercial devices and complex construction, risking equipment damage.
  • The low energy and modest requirements of LTP sources make them suitable for mobile applications and upgrading existing mass analyzers.

Purpose of the Study:

  • To develop a reproducible and accessible low-temperature plasma (LTP) probe for ambient mass spectrometry.
  • To overcome the limitations of commercial LTP devices by utilizing readily available components and 3D printing.
  • To enable solvent-free direct detection and mass spectrometry imaging (MSI) of diverse samples.

Main Methods:

  • Designed and constructed a novel LTP probe using exclusively commercial and 3D-printed components.
  • Integrated the 3D-LTP probe with an ion trap mass analyzer.
  • Evaluated the probe's performance by analyzing pure standards, biological substances, and pharmaceutical samples.

Main Results:

  • The developed LTP probe generates a plasma jet with a ~200 μm diameter, suitable for ambient imaging of macroscopic samples.
  • Successfully detected organic and chemical compounds, primarily as protonated molecules or water/ammonium adducts.
  • Compound identification was confirmed using standard collision-induced dissociation (CID) fragmentation spectra.

Conclusions:

  • The reproducible 3D-LTP probe design facilitates the adoption and advancement of low-temperature plasma technology in analytical chemistry.
  • Open-source availability of design files promotes community-driven development and wider accessibility.
  • This innovation addresses the need for user-friendly and reliable LTP sources for ambient MS applications.