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Related Experiment Video

Updated: Mar 19, 2026

Imaging Intermediate Filaments and Microtubules with 2-dimensional Direct Stochastic Optical Reconstruction Microscopy

Imaging Intermediate Filaments and Microtubules with 2-dimensional Direct Stochastic Optical Reconstruction Microscopy

Published on: March 6, 2018

Focusing super resolution on the cytoskeleton.

Eric A Shelden¹, Zachary T Colburn¹, Jonathan C R Jones¹

¹School of Molecular Biosciences, Washington State University, Pullman, WA, USA.

|June 16, 2016

Summary

This summary is machine-generated.

Super resolution imaging offers advanced cell biology visualization, but challenges remain for live-cell imaging. This review compares super resolution techniques for fixed and live specimens, focusing on cytoskeleton structures.

Keywords:

cytoskeleton live cell imaging microscopy

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Super-resolution Imaging of the Bacterial Division Machinery

Super-resolution Imaging of the Bacterial Division Machinery

Published on: January 21, 2013

Area of Science:

Cell Biology
Microscopy
Biophysics

Background:

Super resolution imaging is a Nobel Prize-winning technique revolutionizing cell biology.
Commercial availability of super resolution instruments accelerates method development.
Super resolution microscopy combines light microscopy benefits with higher resolution.

Purpose of the Study:

To review and compare super resolution imaging systems.
To discuss advantages and disadvantages for fixed and live cell imaging.
To focus on cytoskeleton structure visualization.

Main Methods:

Comparative analysis of super resolution techniques.
Evaluation of suitability for fixed and live specimens.
Assessment of computational demands.

Main Results:

Super resolution microscopy provides high-resolution imaging with gentle sample preparation.
Visualizing dynamic changes in living cells is possible but computationally intensive.
Different super resolution systems have varying strengths for specific applications.

Conclusions:

Super resolution imaging is a powerful tool for cell biology, offering significant advantages.
Challenges in live-cell imaging and computational load require further research.
The choice of super resolution system depends on the specific biological question and specimen type, particularly for cytoskeleton studies.