Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Overview of Nitrogen Metabolism01:20

Overview of Nitrogen Metabolism

12.4K
Nitrogen is a very important element for life because it is a major constituent of proteins and nucleic acids. It is a macronutrient, and in nature, it is recycled from organic compounds and stored in the form of  ammonia, ammonium ions, nitrate, nitrite, or  nitrogen gas by many metabolic processes. Many of these metabolic processes are carried out only by prokaryotes.
The largest pool of nitrogen available in the terrestrial ecosystem is gaseous nitrogen (N2) from the air, but this...
12.4K
Inorganic Nitrogen Assimilation01:22

Inorganic Nitrogen Assimilation

759
Nitrogen is an essential element in biological systems, forming a crucial component of proteins, nucleic acids, and other cellular constituents. Many bacteria and archaea acquire nitrogen in the form of nitrate (NO₃⁻) or ammonia (NH₃), which are then assimilated into biomolecules through specific enzymatic pathways.Assimilatory Nitrate ReductionWhen nitrate enters the cell, it undergoes a two-step reduction process known as assimilatory nitrate reduction. Initially, the enzyme...
759
Cells Coordinate Growth and Proliferation02:36

Cells Coordinate Growth and Proliferation

5.3K
Cell size is a significant factor impacting cellular design, function, and fitness. There exists some internal coordination by which cells double their masses before division, thus, achieving homeostasis. Coordination between cell growth and proliferation depends on the checkpoints in between cell cycle phases. Loss of coordination or failure in the checkpoint mechanism can drive the cell to uncontrolled growth and loss of cellular function. Like dividing cells that coordinate cellular growth,...
5.3K
Cells Coordinate Growth and Proliferation02:36

Cells Coordinate Growth and Proliferation

3.2K
3.2K
The Nitrogen Cycle01:49

The Nitrogen Cycle

61.3K
Nitrogen atoms, present in all proteins and DNA, are recycled between abiotic and biotic components of the ecosystem. However, the primary form of nitrogen on Earth is nitrogen gas, which cannot be used by most animals and plants. Thus, nitrogen gas must first be converted into a usable form by nitrogen-fixing bacteria before it can be cycled through other living organisms. The use of nitrogen-containing fertilizers and animal waste products in human agriculture has greatly influenced the...
61.3K
Stringent Response in E. coli01:23

Stringent Response in E. coli

457
Bacterial growth is closely tied to nutrient availability, with cells proliferating exponentially under favorable conditions and entering a stationary phase when resources become scarce. This transition is mediated by a regulatory mechanism known as the stringent response, which allows bacteria to adapt to nutrient deprivation by modulating gene expression and metabolic activity.During nutrient scarcity, intracellular amino acid levels decline. It results in the accumulation of uncharged tRNAs...
457

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

SLC15A3-mediated dipeptide metabolism confers antimetabolite resistance in lymphoma via mTORC1 activation.

The Journal of clinical investigation·2026
Same author

Heterozygous <i>MMACHC</i> burden variants are associated with higher circulating vitamin B12 in the <i>All of Us</i> Research Program.

medRxiv : the preprint server for health sciences·2026
Same author

Hyperglycosylation is a metabolic driver of Alzheimer's disease.

Nature metabolism·2026
Same author

MitoSAM-dependent lipoylation controls postnatal heart development via metabolic remodeling.

bioRxiv : the preprint server for biology·2026
Same author

Characterizing the metabolomes of microglia, astrocytes and neurons in ageing and Alzheimer's brains.

Nature cell biology·2026
Same author

Nitrogen metabolism profiling reveals cell state-specific pyrimidine synthesis pathway choice.

Nature metabolism·2026

Related Experiment Video

Updated: Mar 19, 2026

Quantifying Tissue-Specific Proteostatic Decline in Caenorhabditis elegans
09:18

Quantifying Tissue-Specific Proteostatic Decline in Caenorhabditis elegans

Published on: September 7, 2021

3.4K

Proliferating Cells Conserve Nitrogen to Support Growth.

Ralph J DeBerardinis1

  • 1Children's Medical Center Research Institute, University of Texas Southwestern Medical Center, Dallas, TX 75390-8502, USA.

Cell Metabolism
|June 16, 2016
PubMed
Summary

Proliferating cells alter glutamate metabolism to conserve nitrogen, demonstrating a direct link between cell growth signals and metabolic pathways. This metabolic shift is crucial for understanding cellular proliferation and nutrient utilization.

More Related Videos

Anaerobic Growth and Maintenance of Mammalian Cell Lines
07:15

Anaerobic Growth and Maintenance of Mammalian Cell Lines

Published on: July 21, 2018

10.8K
Monitoring Protein-Ligand Interactions in Human Cells by Real-Time Quantitative In-Cell NMR using a High Cell Density Bioreactor
10:25

Monitoring Protein-Ligand Interactions in Human Cells by Real-Time Quantitative In-Cell NMR using a High Cell Density Bioreactor

Published on: March 9, 2021

3.9K

Related Experiment Videos

Last Updated: Mar 19, 2026

Quantifying Tissue-Specific Proteostatic Decline in Caenorhabditis elegans
09:18

Quantifying Tissue-Specific Proteostatic Decline in Caenorhabditis elegans

Published on: September 7, 2021

3.4K
Anaerobic Growth and Maintenance of Mammalian Cell Lines
07:15

Anaerobic Growth and Maintenance of Mammalian Cell Lines

Published on: July 21, 2018

10.8K
Monitoring Protein-Ligand Interactions in Human Cells by Real-Time Quantitative In-Cell NMR using a High Cell Density Bioreactor
10:25

Monitoring Protein-Ligand Interactions in Human Cells by Real-Time Quantitative In-Cell NMR using a High Cell Density Bioreactor

Published on: March 9, 2021

3.9K

Area of Science:

  • Cellular metabolism
  • Molecular biology
  • Biochemistry

Background:

  • Glutamate is a key intracellular source of carbon and nitrogen for cellular processes.
  • Cellular proliferation requires significant metabolic adaptation to meet increased demands for biosynthesis and energy.

Purpose of the Study:

  • To investigate how proliferating cells utilize the intracellular glutamate pool.
  • To determine if cellular proliferation influences nitrogen metabolism related to glutamate.

Main Methods:

  • Analysis of cellular metabolic pathways in proliferating cells.
  • Tracking of nitrogen and carbon flux from glutamate.

Main Results:

  • Proliferating cells exhibit a shift in glutamate catabolism.
  • This shift moves from a nitrogen-wasting to a nitrogen-sparing metabolic mode.
  • This highlights the connection between proliferative signals and metabolic regulation.

Conclusions:

  • Cellular proliferation is tightly regulated by metabolic reprogramming.
  • The cell's nitrogen economy is dynamically adjusted during proliferation.
  • Understanding these metabolic shifts is key to understanding cell growth.