Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Cell-surface Signaling

Cell-surface Signaling

Hormones—or any molecule that binds to a receptor, known as a ligand—that are lipid-insoluble (water-soluble) are not able to diffuse across the cell membrane. In order to be able to affect a cell without entering it, these hormones bind to receptors on the cell membrane. When a first messenger, a hormone, binds to a receptor, a signal cascade is set off, causing second messengers, proteins inside the cell, to become activated, resulting in downstream effects.

What is Genetic Engineering?

What is Genetic Engineering?

Types of Receptors: Cell Surface Receptors

Types of Receptors: Cell Surface Receptors

Cell-surface receptors, also known as transmembrane receptors, are cell surface, membrane-anchored (integral) proteins that bind to external ligand molecules. This type of receptor spans the plasma membrane and performs signal transduction, converting an extracellular signal into an intracellular signal. Ligands that interact with cell-surface receptors do not have to enter the cell that they affect. Cell-surface receptors are also called cell-specific proteins or markers because they are...

Surface Tension and Surface Energy

Surface Tension and Surface Energy

When a paint brush is immersed in water, the bristles wave freely inside the water. When it is taken out, the bristles stick together. The reason behind this effect is surface tension.
Consider a beaker filled with liquid. The bulk molecules in the liquid experience equal attractive forces on all sides with the surrounding molecules. However, the surface molecules experience a net attractive force downward due to the bulk molecules. The surface of the liquid behaves like a stretched membrane,...

Heat Engines

Heat Engines

A heat engine is a device used to extract heat from a source and then convert it into mechanical work used for various applications. For example, a steam engine on an old-style train can produce the work needed for driving the train.
Whenever we consider heat engines (and associated devices such as refrigerators and heat pumps), we do not use the standard sign convention for heat and work. For convenience, we assume that the symbols Qh, Qc, and W represent only the amounts of heat transferred...

Zygotic Development And Stem Cell Formation

Zygotic Development And Stem Cell Formation

The development of all multicellular organisms starts with the fusion of haploid cells called sperm and egg to form a diploid zygote. A zygote is a totipotent cell that can develop into a complete organism. The zygote undergoes cell division or cleavage to form an 8-cell mass. Until this stage, the cells are spherical, loosely attached, and remain totipotent. Totipotent cells are capable of developing both the embryonic and the extraembryonic tissues. However, as they continue to divide, they...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Yeast Molecular Display for the Screening and Production of Immunological Proteins Using Nanobody as an Example.

Methods in molecular biology (Clifton, N.J.)·2026

Same author

Autonomous ribosome biogenesis in vitro.

Nature communications·2025

Same author

Bacterial removal using liposomes and an anionic adsorber.

Journal of bioscience and bioengineering·2024

Same author

In vitro selection of dye-fluorescence-enhancing peptide aptamer by cDNA display.

Analytical biochemistry·2024

Same author

Secretion of mitochondrial DNA via exosomes promotes inflammation in Behçet's syndrome.

The EMBO journal·2023

Same author

Application of peptide barcoding to obtain high-affinity anti-PD-1 nanobodies.

Journal of bioscience and bioengineering·2023

Search research articles

Related Experiment Video

Updated: Feb 10, 2026

Protein Engineering by Yeast Surface Display

Protein Engineering by Yeast Surface Display

Published on: November 29, 2024

Establishment of cell surface engineering and its development.

Mitsuyoshi Ueda¹

¹a Division of Applied Life Sciences, Graduate School of Agriculture , Kyoto University , Sakyo-ku , Japan.

Bioscience, Biotechnology, and Biochemistry

|June 16, 2016

Summary

This summary is machine-generated.

Cell surface display technology, or arming technology, utilizes engineered yeasts for protein expression. This powerful method enables efficient protein engineering and diverse industrial applications.

Keywords:

arming technology cell surface engineering engineered yeast protein engineering whole-cell biocatalyst

More Related Videos

Preparation of Thermoresponsive Nanostructured Surfaces for Tissue Engineering

Preparation of Thermoresponsive Nanostructured Surfaces for Tissue Engineering

Published on: March 1, 2016

ECM Protein Nanofibers and Nanostructures Engineered Using Surface-initiated Assembly

ECM Protein Nanofibers and Nanostructures Engineered Using Surface-initiated Assembly

Published on: April 17, 2014

Related Experiment Videos

Last Updated: Feb 10, 2026

Protein Engineering by Yeast Surface Display

Protein Engineering by Yeast Surface Display

Published on: November 29, 2024

Preparation of Thermoresponsive Nanostructured Surfaces for Tissue Engineering

Preparation of Thermoresponsive Nanostructured Surfaces for Tissue Engineering

Published on: March 1, 2016

ECM Protein Nanofibers and Nanostructures Engineered Using Surface-initiated Assembly

ECM Protein Nanofibers and Nanostructures Engineered Using Surface-initiated Assembly

Published on: April 17, 2014

Area of Science:

Biotechnology
Molecular Biology
Synthetic Biology

Background:

Cell surface display involves localizing proteins/peptides to the cell surface.
Conventional intracellular and extracellular expression systems have limitations.
Yeast hosts offer advantages for protein folding control in cell surface display.

Purpose of the Study:

To review strategies for constructing engineered yeasts for cell surface display.
To outline diverse applications of cell surface display technology.
To highlight the utility of cell surface display for protein engineering and directed evolution.

Main Methods:

Engineering yeast hosts for cell surface protein/peptide localization.
Utilizing natural protein folding control in yeast.
Developing high-throughput screening methods for cell surface displayed proteins.

Main Results:

Cell surface display is effective in yeast, facilitating natural protein folding.
Diverse applications exist in biofuels, chemicals, bioremediation, and health.
Direct analysis of intact cells simplifies screening and engineering.

Conclusions:

Cell surface display is a powerful and promising technique for creating functional proteins.
It enables novel protein engineering and directed evolution.
This technology has broad applicability in industrial and research settings.