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Microbial recovery from clot-activating Vacutainers®.

Shangxin Yang1, Ian H McHardy1, Peera Hemarajata1

  • 1Pathology and Laboratory Medicine, UCLA, Los Angeles, CA, USA.

Diagnostic Microbiology and Infectious Disease
|June 17, 2016
PubMed
Summary

BD Vacutainers tubes, commonly used for biological specimen collection, can impact microbial recovery. While most bacteria and yeast showed no significant difference, Haemophilus influenzae recovery was notably reduced in clot activator tubes.

Keywords:
BD VacutainersBacterial recoveryClinical microbiology

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Area of Science:

  • Microbiology
  • Clinical Diagnostics
  • Medical Device Analysis

Background:

  • BD Vacutainers are widely used for biological specimen collection but not optimized for microbial recovery.
  • The impact of standard collection tubes on microbial viability is a critical consideration in diagnostic accuracy.

Purpose of the Study:

  • To evaluate the effect of different BD Vacutainer tube types (glass, plastic, with/without clot activator) on bacterial and fungal recovery.
  • To determine if specific collection tube compositions compromise the recovery of clinically relevant microorganisms.

Main Methods:

  • Bacterial and fungal recovery rates were assessed using both glass and plastic BD Vacutainer tubes.
  • Tubes with and without clot-activating silica were compared to evaluate the influence of clot activators.

Main Results:

  • No significant impact on the recovery of most tested bacteria and yeasts was observed across different tube types.
  • A significant reduction in Haemophilus influenzae recovery was documented in both glass and plastic clot activator tubes compared to controls.

Conclusions:

  • Standard BD Vacutainer tubes generally support the recovery of common bacteria and yeasts.
  • The presence of clot activators in BD Vacutainer tubes negatively affects Haemophilus influenzae recovery from cerebrospinal fluid, necessitating careful consideration in microbiological analysis.