Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Metabolic compartmentation.

H O Spivey, J M Merz

    Bioessays : News and Reviews in Molecular, Cellular and Developmental Biology
    |April 1, 1989
    PubMed
    Summary
    This summary is machine-generated.

    Enzyme associations in vivo are common and offer physiological benefits like substrate channeling. These enzyme complexes, often undetectable in vitro, are crucial for cellular efficiency and function.

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    Substrate channeling.

    Methods (San Diego, Calif.)·1999
    Same author

    Detection of kallikrein gene expression and enzymatic activity in porcine endometrium during the estrous cycle and early pregnancy.

    Biology of reproduction·1999
    Same author

    A computer-controlled variable light attenuator for protection and autoranging of a laser-induced fluorescence detector for capillary zone electrophoresis.

    Electrophoresis·1998
    Same author

    Determination of the specific radioactivity of [14C]lactate by enzymatic decarboxylation and 14CO2 collection.

    Analytical biochemistry·1997
    Same author

    Binding of malate dehydrogenase and NADH channelling to complex I.

    Journal of molecular recognition : JMR·1994
    Same author

    Demonstration of enzyme associations by countermigration electrophoresis in agarose gel.

    Analytical biochemistry·1994

    Area of Science:

    • Biochemistry
    • Molecular Biology
    • Cell Biology

    Background:

    • Soluble enzyme associations are extensively documented in both prokaryotic and eukaryotic cells.
    • In vivo conditions promote enzyme associations that are often too weak to detect in vitro.
    • These associations occur across all cellular compartments.

    Purpose of the Study:

    • To explore the physiological advantages of associated enzyme complexes.
    • To investigate the mechanisms of metabolite microcompartmentation, specifically substrate channeling.
    • To address and refute criticisms regarding the in vivo significance of enzyme associations.

    Main Methods:

    • Analysis of in vivo factors promoting enzyme associations.
    • Investigation of substrate channeling mechanisms: direct transfer and proximity effects.

    Related Experiment Videos

  • Review and theoretical/experimental rebuttal of criticisms concerning enzyme associations.

    • Main Results:

    • Enzyme associations confer significant physiological advantages, primarily through substrate channeling.
    • Substrate channeling can occur via direct transfer or proximity effects, with proximity effects being more general.
    • Criticisms of enzyme association theories have been largely resolved through recent research.

    Conclusions:

    • In vivo enzyme associations are critical for cellular function, offering advantages like enhanced metabolic efficiency.
    • Substrate channeling, facilitated by enzyme proximity, is a key mechanism for metabolite microcompartmentation.
    • While in vitro studies provide foundational insights, in vivo evidence confirms the importance of these enzyme complexes.