Related Experiment Videos
An enhancer in the c-mos locus binds a nuclear factor 1-like protein
F A van der Hoorn1, S K Nordeen
1Department of Pathology, University of Colorado Health Sciences Center, Denver 80262.
Biochemical and Biophysical Research Communications
|May 30, 1989
Summary
Researchers found a DNA enhancer in the c-mos protooncogene that increases transcription. However, a specific binding site for Nuclear Factor 1 (NF-1) within this enhancer is not essential for its function in fibroblasts.
Area of Science:
- Molecular Biology
- Gene Regulation
- Oncogene Research
Background:
- A DNA sequence element in the rat c-mos protooncogene functions as an enhancer.
- This enhancer increases transcription from a heterologous promoter in fibroblasts.
- It operates over large distances and is orientation-independent.
Purpose of the Study:
- To investigate the proteins that bind to the c-mos enhancer.
- To determine the role of Nuclear Factor 1 (NF-1) binding within the enhancer's activity.
Main Methods:
- Identification of nuclear proteins binding to the enhancer.
- Oligonucleotide-directed mutagenesis to alter the NF-1 binding site.
- Assessing enhancer activity after mutagenesis.
- Testing the effect of tumor growth factor-beta on enhancer activity.
Main Results:
- Three mouse nuclear proteins bind to the c-mos enhancer.
- One of these proteins exhibits Nuclear Factor 1 (NF-1) like activity.
- Mutagenesis of the NF-1 binding site abolished protein binding but did not affect enhancer activity.
- Tumor growth factor-beta had no effect on the enhancer's activity.
Conclusions:
- The NF-1 binding site within the c-mos enhancer is not critical for its function in fibroblasts.
- The enhancer's activity is independent of NF-1 binding in this cellular context.