Related Experiment Video
Updated: Mar 19, 2026
11:19
Label-Free Immunoprecipitation Mass Spectrometry Workflow for Large-scale Nuclear Interactome Profiling
Published on: November 17, 2019
17.2K
A convex optimization approach for identification of human tissue-specific interactomes.
Shahin Mohammadi1, Ananth Grama1
1Department of Computer Sciences, Purdue University, West Lafayette, IN 47907, USA.
Bioinformatics (Oxford, England)
|June 17, 2016
Summary
We developed a new method to create tissue-specific interactomes, improving the identification of disease pathways and potential drug targets. This approach enhances understanding of cellular function and disease mechanisms.
Area of Science:
- Bioinformatics
- Systems Biology
- Genomics
Background:
- Organism-specific interactomes offer insights into cellular functions and disease mechanisms.
- Gene expression varies by cell type, influencing tissue-specific functions and pathologies.
- Tissue-specific interactions are crucial for identifying drug targets and understanding disease-related transcription factors.
Purpose of the Study:
- To develop a novel technique for constructing human tissue-specific interactomes.
- To validate the proposed method against state-of-the-art techniques.
- To demonstrate the application of tissue-specific interactomes in identifying disease pathways and drug targets.
Main Methods:
- Construction of human tissue-specific interactomes.
- Validation using Edge Set Enrichment Analysis, Gene Ontology Enrichment, and Disease-Gene Subnetwork Compactness.
- Case studies on Alzheimer's and Parkinson's diseases.
Main Results:
- The novel technique significantly outperforms existing methods.
- Tissue-specific interactomes were successfully constructed.
- Derived interactomes facilitated the construction of pathology-implicated pathways and identification of novel targets.
Conclusions:
- The developed method provides a powerful tool for generating refined tissue-specific interactomes.
- Tissue-specific interactomes are valuable for understanding disease mechanisms and discovering therapeutic targets.
- This approach has significant implications for drug discovery and personalized medicine.
Related Concept Videos
02:04Protein-protein Interfaces
15.0K
Many proteins form complexes to carry out their functions, making protein-protein interactions (PPIs) essential for an organism's survival. Most PPIs are stabilized by numerous weak noncovalent chemical forces. The physical shape of the interfaces determines the way two proteins interact. Many globular proteins have closely-matching shapes on their surfaces, which form a large number of weak bonds. Additionally, many PPIs occur between two helices or between a surface cleft and a...
15.0K
02:26Protein Networks
4.7K
An organism can have thousands of different proteins, and these proteins must cooperate to ensure the health of an organism. Proteins bind to other proteins and form complexes to carry out their functions. Many proteins interact with multiple other proteins creating a complex network of protein interactions.
These interactions can be represented through maps depicting protein-protein interaction networks, represented as nodes and edges. Nodes are circles that are representative of a protein,...
These interactions can be represented through maps depicting protein-protein interaction networks, represented as nodes and edges. Nodes are circles that are representative of a protein,...
4.7K
01:57Protein Complexes with Interchangeable Parts
3.0K
Groups of proteins may form a complex where each protein in this complex has a different role in the overall execution of the complex’s function. Often some of the proteins in the complex can be replaced by a closely related variant to give a complex that contains many of the same components yet is functionally distinct.
The SCF ubiquitin ligase is a protein complex of five individual proteins. This complex attaches ubiquitin to other target proteins to mark them for degradation. In order...
The SCF ubiquitin ligase is a protein complex of five individual proteins. This complex attaches ubiquitin to other target proteins to mark them for degradation. In order...
3.0K
01:57Protein Complexes with Interchangeable Parts
2.2K
2.2K
01:49Conserved Binding Sites
5.3K
Many proteins’ biological role depends on their interactions with their ligands, small molecules that bind to specific locations on the protein known as ligand-binding sites. Ligand-binding sites are often conserved among homologous proteins as these sites are critical for protein function.
Binding sites are often located in large pockets, and if their location on a protein’s surface is unknown, it can be predicted using various approaches. The energetic method computationally...
Binding sites are often located in large pockets, and if their location on a protein’s surface is unknown, it can be predicted using various approaches. The energetic method computationally...
5.3K