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Published on: July 22, 2012
Study on Rotavirus Infection and Its Genotyping in Children Below 5 Years in South West Iran
Azarakhsh Azaran1, Manoochehr Makvandi2, Alireza Samarbafzadeh2
1Virology Department, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, IR Iran.
Insights
Human rotavirus (RV) causes severe diarrhea in young children globally. In Ahvaz, Iran, G1P[8] and G2P[4] were the most common rotavirus genotypes identified in children with acute diarrhea.
Area of Science:
- Virology
- Pediatrics
- Public Health
Background:
- Human rotaviruses are a primary cause of severe dehydrating diarrhea in children under five.
- Rotavirus infection poses a significant global public health challenge.
Purpose of the Study:
- To determine the prevalence of rotavirus infection.
- To identify circulating rotavirus genotypes in children under five with acute diarrhea in Ahvaz, Iran.
Main Methods:
- Collected 200 stool samples from children under five with acute diarrhea (Oct 2011 - Mar 2012).
- Utilized ELISA for initial rotavirus antigen detection.
- Confirmed positive samples and determined G and P genotypes using RT-PCR targeting VP6, VP7, and VP4 genes.
Main Results:
- Rotavirus antigen was detected in 50% of samples by ELISA, with 36.5% confirmed positive by RT-PCR.
- Of the identified strains, 86.3% were typeable for VP7 and VP4.
- The most prevalent rotavirus genotypes were G1P[8] (80%) and G2P[4] (20%).
Conclusions:
- Group A rotavirus is a significant pathogen causing acute diarrhea in Ahvaz.
- The identified rotavirus genotypes align with those found in other international regions.
Background:
Human rotaviruses are the most important agents for severe dehydrating diarrhea in children below 5 years old. Rotaviruses (RV) is a serious public health problem in developing and developed countries.
Objectives:
The aim of this study was to determine the prevalence of rotavirus infection and their genotypes in children younger than 5 years of age with acute diarrhea in Ahvaz, Iran.
Materials And Methods:
For this study, 200 stool samples from children below 5 years of age with acute diarrhea were collected between October 2011 and March 2012. Initially all stool samples were tested for rotavirus antigen by ELISA, and positive samples were confirmed by RT-PCR targeting the VP6 rotavirus gene. Determination of rotavirus genotypes was carried out by performing RT-PCR for G and P types. Altogether, 15 samples were sequenced.
Results:
Out of 200 stool samples, 100 (50%) had rotavirus antigen detected by ELISA and 73 (36.5%) were found positive by RT-PCR. Of the rotavirus strains identified, only 63 (86.3%) were positive for both VP7 and VP4 while 10 (13.7%) strains were found nontypeable. Rotavirus infection accounts for 36.5% of gastroenteritis cases in samples from symptomatic children. The most prevalent rotavirus genotypes were G1P [8] (80%) followed by G2P [4] (20%).
Conclusions:
Our results suggest that group A rotavirus is a major pathogene of acute diarrhea in Ahvaz city. The genotypes circulating are similar with those of other countries.
