Related Experiment Video

Updated: Mar 19, 2026

Immunofluorescence Analysis of Endogenous and Exogenous Centromere-kinetochore Proteins
05:35

Immunofluorescence Analysis of Endogenous and Exogenous Centromere-kinetochore Proteins

Published on: March 3, 2016

15.8K

TRAMM, a new player in CENP-E biology

Miroslav P Milev1, Michael Sacher2

  • 1Concordia University, Department of Biology , Montreal, Quebec, Canada.

Molecular & Cellular Oncology
|June 17, 2016
PubMed

Insights

Centromere-associated protein E (CENP-E) is crucial for mitosis and a cancer therapy target. New research shows TRAMM protein is key in recruiting CENP-E to kinetochores during cell division.

Area of Science:

  • Cell Biology
  • Molecular Biology
  • Cancer Therapeutics

Background:

  • Mitosis is a fundamental cell division process regulated by checkpoints for accurate chromosome distribution.
  • Centromere-associated protein E (CENP-E) is vital during mitosis and represents a promising target for anti-cancer drug development.

Purpose of the Study:

  • To investigate the role of the protein TRAMM (trafficking of membranes and mitosis) in the cellular localization of CENP-E.
  • To understand the molecular mechanisms underlying CENP-E recruitment to kinetochores.

Main Methods:

  • Immunofluorescence microscopy to visualize protein localization.
  • Biochemical assays to study protein interactions.
  • Cell-based assays to assess mitotic progression and chromosome alignment.

Main Results:

  • The protein TRAMM was found to be involved in the recruitment of CENP-E to kinetochores.
  • TRAMM plays a significant role in ensuring proper CENP-E localization during mitosis.

Conclusions:

  • TRAMM is implicated as a critical factor in the kinetochore targeting of CENP-E.
  • Targeting the TRAMM-CENP-E interaction could offer novel therapeutic strategies for cancer treatment.
Keywords:
CENP-ETRAMMTRAPPchromosome congressionmitosis

More Related Videos

Author Spotlight: Establishing CENP-E Knockout HeLa Cells – A Novel Approach to Study Kinesin-7 CENP-E Biology and its Inhibitors11:49

Author Spotlight: Establishing CENP-E Knockout HeLa Cells – A Novel Approach to Study Kinesin-7 CENP-E Biology and its Inhibitors

Published on: June 23, 2023

1.3K
Mass Spectrometry Analysis to Identify Ubiquitylation of EYFP-tagged CENP-A EYFP-CENP-A09:02

Mass Spectrometry Analysis to Identify Ubiquitylation of EYFP-tagged CENP-A EYFP-CENP-A

Published on: June 10, 2020

6.1K

Related Concept Videos

Histone Variants at the Centromere02:30

Histone Variants at the Centromere

Histone variants are the histone proteins with structural and sequence variations. These variants may be regarded as “mutant” forms that replace their canonical histone counterparts in the nucleosomes. Specific post-translational modifications on the histone variants enable further chromatin complexity and regulate tissue-specific gene expression. The most common histone variants are from histone H2A, H2B, and linker histone H1 families. However, several variants of histone H3...
5.2K
Cotranslational Protein Translocation01:20

Cotranslational Protein Translocation

Translocation of proteins across membranes is an ancient process that occurs even in bacteria and archaebacteria. In fact, the components of the translocation machinery are still conserved between prokaryotes and eukaryotes.
Sec61 channel partners for cotranslational translocation
During cotranslational translocation, the Sec61 channel partners with the signal recognition particle (SRP), the signal recognition particle receptor (SR), and the ribosomes to transport the nascent polypeptide chain...
10.9K
Centrosome Duplication02:25

Centrosome Duplication

The primary microtubule organizing center (MTOC) in animal cells is the centrosome. A centrosome has two cylindrical centrioles at its core. Each centriole consists of nine sets of three microtubules held together by proteins. The centrioles are positioned at right angles to each other and surrounded by a shapeless protein cloud called the pericentriolar matrix, or pericentriolar material (PCM).
To ensure that each daughter cell receives a centrosome after cell division, centrosome duplication...
5.1K
Centrosome Duplication02:25

Centrosome Duplication

2.8K
Centrioles and Centrosomes01:13

Centrioles and Centrosomes

Most animal cells comprise a pair of centrioles together called a centrosome. The cell duplicates its centrosome and contains two centrosomes side-by-side, which begin to move apart during the prophase. As the centrosomes migrate to two different sides of the cell, microtubules start extending from each centrosome toward the other end. The mitotic spindle is composed of the centrosomes and their emerging microtubules.
Near the end of the prophase, also called late prophase or...
7.1K
Protein Translocation Machinery on the ER Membrane01:28

Protein Translocation Machinery on the ER Membrane

The translocon complex situated on the ER membrane is the main gateway for the protein secretory pathway. It facilitates the transport of nascent peptides into the ER lumen and their insertion into the ER membrane.
Sec61 protein conducting channel
In eukaryotes, the translocon complex comprises a core heterotrimeric translocator channel called the Sec61 complex. This channel includes three transmembrane proteins, Sec61α, Sec61β, and Sec61γ, and is the largest subunit of the...
7.2K