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Updated: Mar 19, 2026
Absolute Quantification of Aβ1-42 in CSF Using a Mass Spectrometric Reference Measurement Procedure
Published on: March 21, 2017
Development of quantitative mass spectrometric immunoassay for serum amyloid A
Olgica Trenchevska1, Hussein N Yassine2, Chad R Borges1
1a The Biodesign Institute, Arizona State University , Tempe , AZ , USA.
Objective:
Proteins can exist as multiple proteoforms in vivo that can have important roles in physiological and pathological states.
Methods:
We present the development and characterization of mass spectrometric immunoassay (MSIA) for quantitative determination of serum amyloid A (SAA) proteoforms.
Results:
Intra- and inter-day precision revealed CVs <10%. Against existing SAA ELISA, the developed MSIA showed good correlation according to the Altman-Bland plot. Individual concentrations of the SAA proteoforms across a cohort of 170 samples revealed 7 diverse SAA polymorphic types and 12 different proteoforms.
Conclusion:
The new SAA MSIA enables parallel analysis of SAA polymorphisms and quantification of all expressed SAA proteoforms, in a high-throughput and time-efficient manner.
