Fatty acid alters glycogen metabolism in contracting vascular smooth muscle
1Department of Medicine, Rush Medical College, Rush-Presbyterian St. Luke's Medical Center, Chicago, IL 60612.
Abstract:
The purpose of this investigation was to study the effect of fatty acid on carbohydrate metabolism in contracting vascular smooth muscle. The glycogen content of porcine carotid artery incubated in media containing only glucose decreased markedly upon contraction with 80 mM KCl. In contrast, when 0.5 mM palmitate was included there was no decrease in glycogen. Furthermore, the maximal isometric force generated was 22% greater than in the absence of palmitate (P less than 0.001). Stimulation of glycogen phosphorylase a activity with contraction was also enhanced with palmitate. Palmitate had no effect on the levels of the high energy phosphates under any substrate condition. Thus, fatty acid profoundly affects glycogen metabolism and contractility in vascular smooth muscle.
More Related Videos
08:03Glucose Uptake Measurement and Response to Insulin Stimulation in In Vitro Cultured Human Primary Myotubes
Published on: June 25, 2017
08:28Isolation of Primary Patient-specific Aortic Smooth Muscle Cells and Semiquantitative Real-time Contraction Measurements In Vitro
Published on: February 15, 2022
Related Concept Videos
GPCRs Regulate Adenylyl Cylase Activity
Two...
cAMP-dependent Protein Kinase Pathways
Nitric Oxide Signaling Pathway
Smooth Muscle Contraction
The onset of contraction is triggered by an increase in calcium ions within the sarcoplasm, similar to the process in striated muscle. However, smooth muscles have a relatively smaller reservoir of the sarcoplasmic...
Metabolic States of the Body: Fasting and Starvation
Type II Diabetes II: Pathophysiology
