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The interior of a whole and unmodified biological object--the zymogen granule--viewed with a high-resolution X-ray
S S Rothman1, N Iskander, D Attwood
1University of California, San Francisco.
Biochimica Et Biophysica Acta
|June 27, 1989
Abstract:
We report the ability of focused soft X-rays to visualize at spatial resolution well beyond that of the optical microscope (less than 100 nm) the interior of a small, whole biological object without fixation, staining, dehydration or sectioning. Quantitative estimation of its protein content with unique femtogram sensitivity is also reported. The present results represent a significant step towards the goals of natural imaging and chemical mapping of biological structures with soft X-rays.