Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

SDS-PAGE01:27

SDS-PAGE

35.4K
Gel electrophoresis is a method that separates biological macromolecules like nucleic acids or proteins by forcing them to pass through a gel matrix under an electric field.
A variation of gel electrophoresis, termed  polyacrylamide gel electrophoresis (PAGE), is commonly used for separating proteins according to their molecular size by passing them through a polyacrylamide gel. Because of the varying charges associated with amino acid side chains, PAGE can be used to separate intact...
35.4K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

NULISA: a proteomic liquid biopsy platform with attomolar sensitivity and high multiplexing.

Nature communications·2023
Same author

Adherence to tyrosine kinase inhibitor and clinical outcomes in patients with chronic myeloid leukemia.

International immunopharmacology·2023
Same author

Oxaliplatin-induced peripheral neurotoxicity in colorectal cancer patients: mechanisms, pharmacokinetics and strategies.

Frontiers in pharmacology·2023
Same author

Digital light processing 3D printing for microfluidic chips with enhanced resolution via dosing- and zoning-controlled vat photopolymerization.

Microsystems & nanoengineering·2023
Same author

Averaging Strategy for Interpretable Machine Learning on Small Datasets to Understand Element Uptake after Seed Nanotreatment.

Environmental science & technology·2023
Same author

Abnormal functional connectivity in the habenula is associated with subjective hyperarousal state in chronic insomnia disorder.

Frontiers in neurology·2023
Same journal

Immobilization of Ytterbium via Polyphenol Chemistry on Implant Materials for Enhanced Cytocompatibility and Antibacterial Properties.

Langmuir : the ACS journal of surfaces and colloids·2026
Same journal

Electrochemical Oxidation Strategy for Integrated CO<sub>2</sub> Capture and Conversion.

Langmuir : the ACS journal of surfaces and colloids·2026
Same journal

Probing Molecular Structural Changes of Buried Interfaces between Polyethylene and Nylon in Polymer Thin Films after Stretching.

Langmuir : the ACS journal of surfaces and colloids·2026
Same journal

Charge Dependence of Local Hydration Dynamics in Poly(Acrylic Acid) Solutions.

Langmuir : the ACS journal of surfaces and colloids·2026
Same journal

Amphiphilic Lubricants Linked by Hydrogen Bonds Achieve Superlubricity and Enhance Water/Oil Tribological Properties.

Langmuir : the ACS journal of surfaces and colloids·2026
Same journal

Spin Dewetting of Ultrathin Polymer Films.

Langmuir : the ACS journal of surfaces and colloids·2026
See all related articles

Related Experiment Video

Updated: Mar 19, 2026

Rab10 Phosphorylation Detection by LRRK2 Activity Using SDS-PAGE with a Phosphate-binding Tag
08:55

Rab10 Phosphorylation Detection by LRRK2 Activity Using SDS-PAGE with a Phosphate-binding Tag

Published on: December 14, 2017

16.2K

pH-Sensitive Poly(histidine methacrylamide).

Huifeng Wang, Haiyan Wu, Chen-Jung Lee

  • 1Key Laboratory of Carbon Fiber and Functional Polymers, Beijing University of Chemical Technology, Ministry of Education , Beijing 10029, China.

Langmuir : the ACS Journal of Surfaces and Colloids
|June 17, 2016
PubMed
Summary
This summary is machine-generated.

This study introduces a novel polymer, poly(histidine methacrylamide) (PHisMA), with tunable properties. PHisMA exhibits pH-dependent antifouling and metal ion chelation, making it versatile for various applications.

More Related Videos

Synthesis of an Intein-mediated Artificial Protein Hydrogel
15:06

Synthesis of an Intein-mediated Artificial Protein Hydrogel

Published on: January 27, 2014

12.7K
Magnetic and Thermal-sensitive PolyN-isopropylacrylamide-based Microgels for Magnetically Triggered Controlled Release
08:39

Magnetic and Thermal-sensitive PolyN-isopropylacrylamide-based Microgels for Magnetically Triggered Controlled Release

Published on: July 4, 2017

9.6K

Related Experiment Videos

Last Updated: Mar 19, 2026

Rab10 Phosphorylation Detection by LRRK2 Activity Using SDS-PAGE with a Phosphate-binding Tag
08:55

Rab10 Phosphorylation Detection by LRRK2 Activity Using SDS-PAGE with a Phosphate-binding Tag

Published on: December 14, 2017

16.2K
Synthesis of an Intein-mediated Artificial Protein Hydrogel
15:06

Synthesis of an Intein-mediated Artificial Protein Hydrogel

Published on: January 27, 2014

12.7K
Magnetic and Thermal-sensitive PolyN-isopropylacrylamide-based Microgels for Magnetically Triggered Controlled Release
08:39

Magnetic and Thermal-sensitive PolyN-isopropylacrylamide-based Microgels for Magnetically Triggered Controlled Release

Published on: July 4, 2017

9.6K

Area of Science:

  • Polymer Chemistry
  • Materials Science
  • Biomaterials

Background:

  • Zwitterionic polymers offer unique properties for advanced applications.
  • Controlling polymer behavior with external stimuli like pH is crucial for smart materials.

Purpose of the Study:

  • To synthesize and characterize a novel switchable zwitterionic polymer, poly(histidine methacrylamide) (PHisMA).
  • To investigate the pH-dependent antifouling, mechanical, and metal ion chelation properties of PHisMA.

Main Methods:

  • Synthesis of PHisMA polymer.
  • Characterization of polymer properties including antifouling, mechanical, and chelation capabilities.
  • pH-dependent studies of PHisMA hydrogels and surfaces.

Main Results:

  • PHisMA demonstrated switchable states (zwitterionic, anionic, cationic) with pH.
  • PHisMA surfaces showed effective antifouling properties against protein adsorption and bacterial attachment at pH 5.
  • PHisMA hydrogels exhibited pH-dependent mechanical properties and swelling behavior.
  • PHisMA effectively chelated multivalent metal ions (Ca2+, Mg2+, Cu2+, Ni2+, Fe3+).

Conclusions:

  • PHisMA is a versatile switchable zwitterionic polymer with tunable antifouling and metal ion binding properties.
  • The study enhances understanding of structure-property relationships in switchable zwitterionic polymers.
  • PHisMA shows significant potential for applications in wound care, water treatment, bioseparation, and drug/gene delivery.