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Updated: Mar 19, 2026

A Computational Pipeline for Intergenic/Intragenic Enhancer RNA Quantification in Mouse Embryonic Stem Cells
Published on: October 28, 2025
Use of Read-Across Tools
Serena Manganelli1, Emilio Benfenati2
1Laboratory of Environmental Chemistry and Toxicology, IRCCS-Istituto di Ricerche Farmacologiche Mario Negri, Via La Masa, 19, 20156, Milan, Italy. serena.manganelli@marionegri.it.
Abstract:
Read-across has become popular since the introduction of regulations, such as the European REACH regulation. This chapter provides instructions on how to use ToxRead, new freely available software for read-across analysis, and on how to interpret its output predictions for mutagenicity assessments.This tool offers two seminal sources: a set of rules/structural alerts, which may explain the toxicity, and a similarity tool, associated with a large database of chemicals with their properties.
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